Protection of chickens against hepatitis-hydropericardium syndrome and Newcastle disease with a recombinant Newcastle disease virus vaccine expressing the fowl adenovirus serotype 4 fiber-2 protein.

Autor: Tian KY; College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, Henan, China., Guo HF; College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, Henan, China., Li N; College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, Henan, China., Zhang YH; College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, Henan, China., Wang Z; College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, Henan, China., Wang B; College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, Henan, China., Yang X; College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, Henan, China., Li YT; College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, Henan, China., Zhao J; College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, Henan, China. Electronic address: zhaoj@henau.edu.cn.
Jazyk: angličtina
Zdroj: Vaccine [Vaccine] 2020 Feb 18; Vol. 38 (8), pp. 1989-1997. Date of Electronic Publication: 2020 Jan 14.
DOI: 10.1016/j.vaccine.2020.01.006
Abstrakt: Newcastle disease (ND) is one of the most important and devastating avian diseases with considerable threat to the global poultry industry. Hepatitis-hydropericardium syndrome (HHS), caused by virulent fowl adenovirus serotype 4 (FAdV-4), is another highly infectious disease in chickens with severe economic impact. The effective way to combat ND and HHS is by vaccinating the poultry. In the present study, a recombinant NDV LaSota vaccine strain expressing full length fiber-2 gene of FAdV-4 (rLaSota-fiber2) was generated using reverse genetics. The FAdV-4 fiber-2 protein was expressed as a soluble form rather than NDV membrane-anchored form. The rLaSota-fiber2 was genetically stable, and it showed growth patterns in embryonated eggs comparable to that of parental rLaSota virus. Since our unpublished data demonstrated that delivery of live rLaSota-fiber2 in drinking water or ocular delivery of the vaccine didn't produce protection against hypervirulent FAdV-4 challenge, even though the vaccine provide full protection against NDV challenge, the efficacy of the rLaSota-fiber2 was evaluated by delivering the vaccine intramuscularly in this study. Single-dose intramuscular vaccination of 2-week-old SPF White Leghorn chicks with the live or inactivated rLaSota-fiber2 provided complete protection against virulent NDV challenge. However, single-dose intramuscular vaccination with the live rLaSota-fiber2 vaccine provided better protection against virulent FAdV-4 challenge and significantly reduced faecal viral shedding comparing to the inactivated vaccine. These results indicate that the NDV-vectored FAdV-4 vaccine is a promising bivalent vaccine candidate to control both HHS and ND.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2020 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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