| Autor: |
Pantic I; Faculty of Medicine, Institute of Medical Physiology, University of Belgrade, Visegradska 26/II, RS-11129Belgrade, Serbia.; University of Haifa, 199 Abba Hushi Blvd., Mount Carmel, Haifa, IL-3498838, Israel., Jeremic R; Faculty of Medicine, Institute of Medical Physiology, University of Belgrade, Visegradska 26/II, RS-11129Belgrade, Serbia., Dacic S; Department for General Physiology and Biophysics, Institute of Physiology and Biochemistry 'Ivan Djaja', Faculty of Biology, University of Belgrade, Studentski trg 16, 11000Belgrade, Serbia., Pekovic S; Department of Neurobiology, Institute for Biological Research 'Sinisa Stankovic'- National Institute of Republic of Serbia, University of Belgrade, Blvd despota Stefana 142, Belgrade, Serbia., Pantic S; School of Medicine, Institute of Histology and Embryology, University of Belgrade, Visegradska 26/II, RS-11129Belgrade, Serbia., Djelic M; Faculty of Medicine, Institute of Medical Physiology, University of Belgrade, Visegradska 26/II, RS-11129Belgrade, Serbia., Vitic Z; Department of Physiology and Cell Biology, Zlotowski Center for Neuroscience, Faculty of Health Sciences, Ben-Gurion University of the Negev, Beersheba, Israel., Brkic P; Faculty of Medicine, Institute of Medical Physiology, University of Belgrade, Visegradska 26/II, RS-11129Belgrade, Serbia., Brodski C; Department of Physiology and Cell Biology, Zlotowski Center for Neuroscience, Faculty of Health Sciences, Ben-Gurion University of the Negev, Beersheba, Israel. |
| Abstrakt: |
Traumatic brain injury (TBI) is a main cause of death and disabilities in young adults. Although learning and memory impairments are a major clinical manifestation of TBI, the consequences of TBI on the hippocampus are still not well understood. In particular, how lesions to the sensorimotor cortex damage the hippocampus, to which it is not directly connected, is still elusive. Here, we study the effects of sensorimotor cortex ablation (SCA) on the hippocampal dentate gyrus, by applying a highly sensitive gray-level co-occurrence matrix (GLCM) analysis. Using GLCM analysis of granule neurons, we discovered, in our TBI paradigm, subtle changes in granule cell (GC) morphology, including textual uniformity, contrast, and variance, which is not detected by conventional microscopy. We conclude that sensorimotor cortex trauma leads to specific changes in the hippocampus that advance our understanding of the cellular underpinnings of cognitive impairments in TBI. Moreover, we identified GLCM analysis as a highly sensitive method to detect subtle changes in the GC layers that is expected to significantly improve further studies investigating the impact of TBI on hippocampal neuropathology. |
