Sex and Depot Differences in Palmitoleic Acid Content of Human Blood and Fat.

Autor: Ramos P; Endocrine Research Unit, Mayo Clinic, 200 1st Street SW, Rm 5-194 Joseph, Rochester, MN, 55905, USA., Bush NC; Endocrine Research Unit, Mayo Clinic, 200 1st Street SW, Rm 5-194 Joseph, Rochester, MN, 55905, USA., Jensen MD; Endocrine Research Unit, Mayo Clinic, 200 1st Street SW, Rm 5-194 Joseph, Rochester, MN, 55905, USA.
Jazyk: angličtina
Zdroj: Lipids [Lipids] 2020 Jan; Vol. 55 (1), pp. 63-72. Date of Electronic Publication: 2020 Jan 16.
DOI: 10.1002/lipd.12212
Abstrakt: Palmitoleic acid has been classified as an insulin-sensitizing lipokine, but evidence for this from human studies has been inconsistent. We hypothesized that this is related to either the types of samples or conditions under which samples are collected. We measured plasma palmitoleic acid and total free fatty acids (FFA) using ultra-performance liquid chromatography in blood samples collected from 34 adults under a variety of conditions. We collected duplicate samples of adipose (n = 10), FFA (n = 9), and very low density lipoprotein triacylglycerol (VLDL-TAG) (n = 7) to measure the palmitoleic acid as a percentage of total fatty acids. We tested whether the percentage of palmitoleic acid was correlated with insulin resistance, as measured by homeostatic model of insulin resistance (HOMA-IR). Adipose stearoyl-coenzyme A desaturase 1 (SCD-1) protein was measured by capillary Western blotting. FFA-palmitoleic acid percentage increased as a function of total FFA and was greater (p < 0.005) in females than males. Adipose palmitoleic acid percentage was greater in females than males (p < 0.001), as was adipose SCD-1. Palmitoleic acid was greater in femoral fat than in abdominal fat in both females and males (p < 0.001), and correlated positively with HOMA-IR only in females. The test-retest reliability values for percentage palmitoleic acid were 7 ± 10% for adipose, 24 ± 26% for VLDL, and 53 ± 31% for FFA. Because FFA-palmitoleic acid percentage varies as a function of total FFA, investigators should re-evaluate how palmitoleic acid data is presented. The positive relationship between adipose palmitoleic acid and HOMA-IR in females suggests that it is not a potent insulin-sensitizing lipokine in humans.
(© 2020 AOCS.)
Databáze: MEDLINE
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