Synthetic Polymers Provide a Robust Substrate for Functional Neuron Culture.

Autor: Zhang Y; School of Chemistry, Kings Buildings, The University of Edinburgh, Edinburgh, EH9 3FJ, UK.; Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, 518055, China., Venkateswaran S; School of Chemistry, Kings Buildings, The University of Edinburgh, Edinburgh, EH9 3FJ, UK., Higuera GA; Department of Neuroscience, Erasmus MC Rotterdam, Rotterdam, NL-3015 GE, The Netherlands., Nath S; Department of Biomechanical Engineering, Delft University of Technology (TU Delft), Mekelweg 2, 2628CD, Delft, The Netherlands., Shpak G; Department of Psychiatry, Erasmus MC Rotterdam, Rotterdam, NL-3015 GE, The Netherlands.; Department of Life Sciences, Erasmus University College, Rotterdam, 3011 HP, The Netherlands., Matray J; Department of Biomechanical Engineering, Delft University of Technology (TU Delft), Mekelweg 2, 2628CD, Delft, The Netherlands., Fratila-Apachitei LE; Department of Biomechanical Engineering, Delft University of Technology (TU Delft), Mekelweg 2, 2628CD, Delft, The Netherlands., Zadpoor AA; Department of Biomechanical Engineering, Delft University of Technology (TU Delft), Mekelweg 2, 2628CD, Delft, The Netherlands., Kushner SA; Department of Biomechanical Engineering, Delft University of Technology (TU Delft), Mekelweg 2, 2628CD, Delft, The Netherlands., Bradley M; School of Chemistry, Kings Buildings, The University of Edinburgh, Edinburgh, EH9 3FJ, UK., De Zeeuw CI; Department of Neuroscience, Erasmus MC Rotterdam, Rotterdam, NL-3015 GE, The Netherlands.; Institute for Neuroscience, Royal Netherlands Academy of Arts and Sciences, Amsterdam, 1105 BA, The Netherlands.
Jazyk: angličtina
Zdroj: Advanced healthcare materials [Adv Healthc Mater] 2020 Feb; Vol. 9 (4), pp. e1901347. Date of Electronic Publication: 2020 Jan 15.
DOI: 10.1002/adhm.201901347
Abstrakt: Substrates for neuron culture and implantation are required to be both biocompatible and display surface compositions that support cell attachment, growth, differentiation, and neural activity. Laminin, a naturally occurring extracellular matrix protein is the most widely used substrate for neuron culture and fulfills some of these requirements, however, it is expensive, unstable (compared to synthetic materials), and prone to batch-to-batch variation. This study uses a high-throughput polymer screening approach to identify synthetic polymers that supports the in vitro culture of primary mouse cerebellar neurons. This allows the identification of materials that enable primary cell attachment with high viability even under "serum-free" conditions, with materials that support both primary cells and neural progenitor cell attachment with high levels of neuronal biomarker expression, while promoting progenitor cell maturation to neurons.
(© 2020 The Authors. Published by WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.)
Databáze: MEDLINE
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