Real-time monitoring of bacterial growth kinetics in suspensions using laser speckle imaging.

Autor: Loutfi H; Physics Department, UR TVA, Faculty of Science, Saint Joseph University, B.P. 11-514, Riad El Solh Beirut, 1107 2050, Lebanon. hadi.loutfi@net.usj.edu.lb.; Laboratoire OPTIMAG, IBSAM, Université de Bretagne Occidentale, 6 avenue Le Gorgeu, C.S. 93837, 29238, Brest Cedex, 3, France. hadi.loutfi@net.usj.edu.lb., Pellen F; Laboratoire OPTIMAG, IBSAM, Université de Bretagne Occidentale, 6 avenue Le Gorgeu, C.S. 93837, 29238, Brest Cedex, 3, France., Le Jeune B; Laboratoire OPTIMAG, IBSAM, Université de Bretagne Occidentale, 6 avenue Le Gorgeu, C.S. 93837, 29238, Brest Cedex, 3, France., Lteif R; Chemistry Department, UR TVA, Faculty of Science, Saint Joseph University, B.P. 11-514, Riad El Solh Beirut, 1107 2050, Lebanon., Kallassy M; Faculty of Science, Biotechnology Laboratory, UR EGP, Saint Joseph University, B.P. 11-514, Riad El Solh Beirut, 1107 2050, Lebanon., Le Brun G; Laboratoire OPTIMAG, IBSAM, Université de Bretagne Occidentale, 6 avenue Le Gorgeu, C.S. 93837, 29238, Brest Cedex, 3, France. guy.lebrun@univ-brest.fr., Abboud M; Physics Department, UR TVA, Faculty of Science, Saint Joseph University, B.P. 11-514, Riad El Solh Beirut, 1107 2050, Lebanon. marie.abboud@usj.edu.lb.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2020 Jan 15; Vol. 10 (1), pp. 408. Date of Electronic Publication: 2020 Jan 15.
DOI: 10.1038/s41598-019-57281-2
Abstrakt: In microbiology, monitoring the growth of any microorganism in culture is important for studying and optimizing the growth kinetics, the biomass and the metabolite production. In this work, we show that laser speckle imaging is a reliable technique that can be used to perform real-time monitoring of bacteria growth kinetic in liquid culture media. Speckle parameters, specifically speckle grain size and the spatial contrast of the speckle images, and standard analytical parameters (optical density, pH and colony forming units) were measured during the culture of different strains of Bacillus thuringiensis. Our results show that both speckle grain size and spatial contrast decrease with bacterial growth. Furthermore, speckle parameters are sensitive to the fermentation conditions. Statistical analysis revealed a relatively high correlation between speckle and analytical parameters.
Databáze: MEDLINE
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