Polypropylene mesh for hernia repair with controllable cell adhesion/de-adhesion properties.

Autor: Lanzalaco S; Departament d'Enginyeria Química, EEBE, Universitat Politècnica de Catalunya, C/Eduard Maristany, 10-14, Ed. I.2, Barcelona, 08019, Spain. sonia.lanzalaco@upc.edu elaine.armelin@upc.edu and Barcelona Research Center for Multiscale Science and Engineering, Universitat Politècnica de Catalunya, C/Eduard Maristany, 10-14, Ed. I.S, Barcelona, 08019, Spain., Del Valle LJ; Departament d'Enginyeria Química, EEBE, Universitat Politècnica de Catalunya, C/Eduard Maristany, 10-14, Ed. I.2, Barcelona, 08019, Spain. sonia.lanzalaco@upc.edu elaine.armelin@upc.edu and Barcelona Research Center for Multiscale Science and Engineering, Universitat Politècnica de Catalunya, C/Eduard Maristany, 10-14, Ed. I.S, Barcelona, 08019, Spain., Turon P; Departament d'Enginyeria Química, EEBE, Universitat Politècnica de Catalunya, C/Eduard Maristany, 10-14, Ed. I.2, Barcelona, 08019, Spain. sonia.lanzalaco@upc.edu elaine.armelin@upc.edu and Research and Development, B. Braun Surgical, S.A. Carretera de Terrassa 121, 08191 Rubí (Barcelona), Spain., Weis C; Departament d'Enginyeria Química, EEBE, Universitat Politècnica de Catalunya, C/Eduard Maristany, 10-14, Ed. I.2, Barcelona, 08019, Spain. sonia.lanzalaco@upc.edu elaine.armelin@upc.edu and Research and Development, B. Braun Surgical, S.A. Carretera de Terrassa 121, 08191 Rubí (Barcelona), Spain., Estrany F; Departament d'Enginyeria Química, EEBE, Universitat Politècnica de Catalunya, C/Eduard Maristany, 10-14, Ed. I.2, Barcelona, 08019, Spain. sonia.lanzalaco@upc.edu elaine.armelin@upc.edu and Barcelona Research Center for Multiscale Science and Engineering, Universitat Politècnica de Catalunya, C/Eduard Maristany, 10-14, Ed. I.S, Barcelona, 08019, Spain., Alemán C; Departament d'Enginyeria Química, EEBE, Universitat Politècnica de Catalunya, C/Eduard Maristany, 10-14, Ed. I.2, Barcelona, 08019, Spain. sonia.lanzalaco@upc.edu elaine.armelin@upc.edu and Barcelona Research Center for Multiscale Science and Engineering, Universitat Politècnica de Catalunya, C/Eduard Maristany, 10-14, Ed. I.S, Barcelona, 08019, Spain., Armelin E; Departament d'Enginyeria Química, EEBE, Universitat Politècnica de Catalunya, C/Eduard Maristany, 10-14, Ed. I.2, Barcelona, 08019, Spain. sonia.lanzalaco@upc.edu elaine.armelin@upc.edu and Barcelona Research Center for Multiscale Science and Engineering, Universitat Politècnica de Catalunya, C/Eduard Maristany, 10-14, Ed. I.S, Barcelona, 08019, Spain.
Jazyk: angličtina
Zdroj: Journal of materials chemistry. B [J Mater Chem B] 2020 Feb 07; Vol. 8 (5), pp. 1049-1059. Date of Electronic Publication: 2020 Jan 15.
DOI: 10.1039/c9tb02537e
Abstrakt: Herein, a versatile bilayer system, composed by a polypropylene (PP) mesh and a covalently bonded poly(N-isopropylacrylamide) (PNIPAAm) hydrogel, is reported. The cell adhesion mechanism was successfully modulated by controlling the architecture of the hydrogel in terms of duration of PNIPAAm grafting time, crosslinker content, and temperature of material exposure in PBS solutions (below and above the LCST of PNIPAAm). The best in vitro results with fibroblast (COS-1) and epithelial (MCF-7) cells was obtained with a mesh modified with a porous iPP-g-PNIPAAm bilayer system, prepared via PNIPAAm grafting for 2 h at the lowest N,N'-methylene bis(acrylamide) (MBA) concentration (1 mM). Under these conditions, the detachment of the fibroblast-like cells was 50% lower than that of the control, after 7 days of cell incubation, which represents a high de-adhesion of cells in a short period. Moreover, the whole system showed excellent stability in dry or wet media, proving that the thermosensitive hydrogel was well adhered to the polymer surface, after PP fibre activation by cold plasma. This study provides new insights on the development of anti-adherent meshes for abdominal hernia repair.
Databáze: MEDLINE
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