| Autor: |
Griffin DM; Interdepartmental Program in Translation Biology and Molecular Medicine, Baylor College of Medicine, Houston, TX, USA., Bitner BR; Interdepartmental Program in Translation Biology and Molecular Medicine, Baylor College of Medicine, Houston, TX, USA., Criss Ii Z; Interdepartmental Program in Translation Biology and Molecular Medicine, Baylor College of Medicine, Houston, TX, USA., Marcano D; Department of Chemistry, Rice University, Houston, TX, USA.; Smalley-Curl Institute for and Nanocarbon Center, Rice University, Houston, TX, USA., Berlin JM; Department of Chemistry, Rice University, Houston, TX, USA.; Smalley-Curl Institute for and Nanocarbon Center, Rice University, Houston, TX, USA.; Molecular Medicine, City of Hope, Duarte, CA, USA., Kent TA; Interdepartmental Program in Translation Biology and Molecular Medicine, Baylor College of Medicine, Houston, TX, USA.; Department of Neurology, Baylor College of Medicine, Houston, TX, USA.; Center for Translational Research on Inflammatory Diseases, Michael E. DeBakey VA Medical Center, Houston, TX, USA., Tour JM; Department of Chemistry, Rice University, Houston, TX, USA.; Smalley-Curl Institute for and Nanocarbon Center, Rice University, Houston, TX, USA., Samson SL; Department of Chemistry, Rice University, Houston, TX, USA.; Department of Medicine, Baylor College of Medicine, Houston, TX, USA., Pautler RG; Interdepartmental Program in Translation Biology and Molecular Medicine, Baylor College of Medicine, Houston, TX, USA.; Department of Molecular Physiology and Biophysics, Baylor College of Medicine, Houston, TX, USA. |
| Abstrakt: |
Background : Oxidative stress has been implicated in metabolic syndrome (MetS); however, antioxidants such as vitamin E have had limited success in the clinic. This prompts the question of what effects amore potent antioxidant might produce. A prime candidate is the recently developed bioengineered antioxidant, poly(ethylene glycol)-functionalizedhydrophilic carbon clusters (PEG-HCCs), which are capable of neutralizing the reactive oxygen species (ROS) superoxide anion and hydroxyl radical at10 6 /molecule of PEG-HCC. In this project, we tested the potential of PEG-HCCs as a possible therapeutic for MetS. Results : PEG-HCC treatment lessened lipid peroxidation, aspartate aminotransferase levels, non-fastingblood glucose levels, and JNK phosphorylation inob/ob mice. PEG-HCC-treated WT mice had an increased response to insulin by insulin tolerance tests and adecrease in blood glucose by glucose tolerance tests. These effects were not observed in HFD-fed mice, regardless of treatment. PEG-HCCs were observed in the interstitial space of liver, spleen, skeletal muscle, and adipose tissue. No significant difference was shown in gluconeogenesis or inflammatory gene expression between treatment and dietary groups. Expert Opinion : PEG-HCCs improved some parameters of disease possibly due to a resulting increase in peripheral insulin sensitivity. However, additional studies are needed to elucidate how PEG-HCCsare producing these effects. |
