Transport of Ca 2+ and Ca 2+ -Dependent Permeability Transition in the Liver and Heart Mitochondria of Rats with Different Tolerance to Acute Hypoxia.

Autor: Belosludtsev KN; Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Institutskaya 3, Pushchino, 142290 Moscow Region, Russia.; Department of Biochemistry, Cell Biology and Microbiology, Mari State University, pl. Lenina 1, Yoshkar-Ola, 424001 Mari El, Russia., Dubinin MV; Department of Biochemistry, Cell Biology and Microbiology, Mari State University, pl. Lenina 1, Yoshkar-Ola, 424001 Mari El, Russia., Talanov EY; Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Institutskaya 3, Pushchino, 142290 Moscow Region, Russia., Starinets VS; Department of Biochemistry, Cell Biology and Microbiology, Mari State University, pl. Lenina 1, Yoshkar-Ola, 424001 Mari El, Russia., Tenkov KS; Department of Biochemistry, Cell Biology and Microbiology, Mari State University, pl. Lenina 1, Yoshkar-Ola, 424001 Mari El, Russia., Zakharova NM; Institute of Cell Biophysics, Russian Academy of Sciences, PSCBR RAS, Institutskaya 3, Pushchino, 142290 Moscow Region, Russia., Belosludtseva NV; Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Institutskaya 3, Pushchino, 142290 Moscow Region, Russia.
Jazyk: angličtina
Zdroj: Biomolecules [Biomolecules] 2020 Jan 09; Vol. 10 (1). Date of Electronic Publication: 2020 Jan 09.
DOI: 10.3390/biom10010114
Abstrakt: The work examines the kinetic parameters of Ca 2+ uptake via the mitochondrial calcium uniporter complex (MCUC) and the opening of the Ca 2+ -dependent permeability transition pore (MPT pore) in the liver and heart mitochondria of rats with high resistance (HR) and low resistance (LR) to acute hypoxia. We found that the rate of Ca 2+ uptake by mitochondria of the liver and heart in HR rats is higher than that in LR rats, which is associated with a higher level of the channel-forming subunit MCU in liver mitochondria of HR rats and a lower content of the dominant-negative channel subunit MCUb in heart mitochondria of HR rats. It was shown that the liver mitochondria of HR rats are more resistant to the induction of the MPT pore than those of LR rats (the calcium retention capacity of liver mitochondria of HR rats was found to be 1.3 times greater than that of LR rats). These data correlate with the fact that the level of F 0 F 1 -ATP synthase, a possible structural element of the MPT pore, in the liver mitochondria of HR rats is lower than in LR rats. In heart mitochondria of rats of the two phenotypes, no statistically significant difference in the formation of the MPT pore was revealed. The paper discusses how changes in the expression of the MCUC subunits and the putative components of the MPT pore can affect Ca 2+ homeostasis of mitochondria in animals with originally different tolerance to hypoxia and in hypoxia-induced tissue injury.
Databáze: MEDLINE
Externí odkaz: