| Autor: |
Salazar-Magallón JA; Colegio de Postgraduados Campus Puebla, Unidad en Desarrollo para la Investigación y Transferencia de Tecnología en Control Biológico, San Pedro Cholula, México., Huerta de la Peña A; Colegio de Postgraduados Campus Puebla, Unidad en Desarrollo para la Investigación y Transferencia de Tecnología en Control Biológico, San Pedro Cholula, México. |
| Jazyk: |
angličtina |
| Zdroj: |
Preparative biochemistry & biotechnology [Prep Biochem Biotechnol] 2020; Vol. 50 (2), pp. 204-214. Date of Electronic Publication: 2020 Jan 14. |
| DOI: |
10.1080/10826068.2019.1676781 |
| Abstrakt: |
Biotechnology through plant cell cultures in bioreactors is a tool that allows increasing the production of secondary metabolites of commercial interest. The hydrodynamic characterization, in addition to the transfer (OTR) and uptake (OUR) of oxygen through the dynamic method with different aeration rate, were used to see their influence on the production of biomass and saponins. The culture poisoning technique was used to determine the antifungal activity of the SC-2 and SC-3 saponins in vitro . Likewise, the shear or hydrodynamic stress of 273.6 mN/m 2 were calculated based on the Reynolds Number. The oxygen supply (OTR) was always greater than the demand (OUR) for all the aeration rate evaluated. Dry weight values of 8.6 gDW/L and a concentration of 2.7 mg/L and 187.3 mg/L of the saponins SC-2 and SC-3 respectively were obtained with an air flow of 0.1 vvm. In addition, it was possible to inhibit the growth of phytopathogenic fungi in vitro by up to 93%, while in vivo it was possible to reduce the infections of strawberry seeds inoculated with phytopathogens, obtaining up to 94% of germinated seeds. This information will facilitate the rational operation of the bioreactor culture system that produces secondary metabolites. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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