| Autor: |
Karama M; Veterinary Public Health Section, Department of Paraclinical Sciences, Faculty of Veterinary Science, University of Pretoria, Onderstepoort, South Africa., Kambuyi K; Veterinary Public Health Section, Department of Paraclinical Sciences, Faculty of Veterinary Science, University of Pretoria, Onderstepoort, South Africa., Cenci-Goga BT; Laboratorio di Ispezione degli Alimenti di Origine Animale, Dipartimento di Medicina Veterinaria, University of Perugia, Perugia, Italy., Malahlela M; Veterinary Public Health Section, Department of Paraclinical Sciences, Faculty of Veterinary Science, University of Pretoria, Onderstepoort, South Africa., Jonker A; Department of Tropical Diseases, Faculty of Veterinary Science, University of Pretoria, Onderstepoort, South Africa., He C; College of Life Science and Engineering, Foshan University, Foshan, China.; Key Laboratory of Animal Epidemiology and Zoonosis of Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University, Beijing, China., Ombui J; Department of Public Health, Pharmacology and Toxicology, College of Agriculture and Veterinary Sciences, University of Nairobi, Nairobi, Kenya., Tshuma T; Department of Production Animal Studies, Faculty of Veterinary Science, University of Pretoria, Onderstepoort, South Africa., Etter E; Department of Production Animal Studies, Faculty of Veterinary Science, University of Pretoria, Onderstepoort, South Africa.; Centre de Coopération Internationale en Recherche Agronomique pour le Développement-INRA, UMR ASTRE Baillarguet International Campus, University of Montpellier, Montpellier, France., Kalake A; Gauteng Department of Agriculture and Rural Development, Johannesburg, South Africa. |
| Abstrakt: |
This study investigated occurrence and antimicrobial resistance profiles of Campylobacter spp. isolates in beef cattle on five cow-calf operations in South Africa. A total of 537 fecal samples from adult beef cattle ( n = 435) and rectal swabs from calves ( n = 102) were screened for Campylobacter jejuni , Campylobacter coli , and Campylobacter upsaliensis by culture and polymerase chain reaction. Furthermore, 86 Campylobacter spp. isolates including 46 C. jejuni , 24 C. coli , and 16 C. upsaliensis were tested for antimicrobial resistance against a panel of 9 antimicrobials. Overall, Campylobacter spp. was detected in 29.7% of cattle. Among the 158 Campylobacter spp.-positive cattle, 61.8% carried C. jejuni , 25% carried C. coli , and 10% carried C. upsaliensis . Five animals (3.1%) had mixed infections: three cows carried C. jejuni and C. coli concurrently, one cow had both C. jejuni and C. upsaliensis , and one cow harbored C. coli and C. upsaliensis . Antimicrobial resistance profiling among 86 Campylobacter spp. isolates revealed that 52.3% of the isolates were resistant to one or more antimicrobials. Antimicrobial resistance was observed in 46.7% of C. jejuni isolates, 35.6% of C. coli , and 17.8% of C. upsaliensis . Thirty-six percent of isolates were resistant to clindamycin, 19.7% to nalidixic acid, 18.6% to tetracycline, and 17.4% to erythromycin. Lower resistance rates were recorded for azithromycin (8.1%), florfenicol (3.4%), gentamicin (4.8%), and telithromycin and ciprofloxacin (5.8%). Multidrug resistance (MDR) was observed in 32.5% of isolates. Significantly higher levels of MDR were detected among C. jejuni (36.9%) and C. coli (33.3%) isolates in comparison to C. upsaliensis (18.7%). Two main multiresistance patterns were detected: nalidixic acid/clindamycin (17.8%) and tetracycline/clindamycin (14.2%). To the best of our knowledge, this is the first study which has shown that beef cattle on cow-calf operations in South Africa constitute an important reservoir and a potential source of clinically relevant and antimicrobial resistant Campylobacter spp. strains. |
