Schizosaccharomyces pombe DNA translocases Rrp1 and Rrp2 have distinct roles at centromeres and telomeres that ensure genome stability.
| Autor: | Barg-Wojas A; Faculty of Biotechnology, University of Wrocław, 50-383 Wrocław, Poland., Muraszko J; Faculty of Biotechnology, University of Wrocław, 50-383 Wrocław, Poland., Kramarz K; Institut Curie, Centre National de la Recherche Scientifique, F-91405, Orsay, France., Schirmeisen K; Faculty of Biotechnology, University of Wrocław, 50-383 Wrocław, Poland., Baranowska G; Faculty of Biotechnology, University of Wrocław, 50-383 Wrocław, Poland., Carr AM; Genome Damage and Stability Centre, School of Life Sciences, University of Sussex, Brighton BN1 9RQ, UK., Dziadkowiec D; Faculty of Biotechnology, University of Wrocław, 50-383 Wrocław, Poland dorota.dziadkowiec@uwr.edu.pl. |
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| Jazyk: | angličtina |
| Zdroj: | Journal of cell science [J Cell Sci] 2020 Feb 10; Vol. 133 (3). Date of Electronic Publication: 2020 Feb 10. |
| DOI: | 10.1242/jcs.230193 |
| Abstrakt: | The regulation of telomere and centromere structure and function is essential for maintaining genome integrity. Schizosaccharomyces pombe Rrp1 and Rrp2 are orthologues of Saccharomyces cerevisiae Uls1, a SWI2/SNF2 DNA translocase and SUMO-targeted ubiquitin ligase. Here, we show that Rrp1 or Rrp2 overproduction leads to chromosome instability and growth defects, a reduction in global histone levels and mislocalisation of centromere-specific histone Cnp1. These phenotypes depend on putative DNA translocase activities of Rrp1 and Rrp2, suggesting that Rrp1 and Rrp2 may be involved in modulating nucleosome dynamics. Furthermore, we confirm that Rrp2, but not Rrp1, acts at telomeres, reflecting a previously described interaction between Rrp2 and Top2. In conclusion, we identify roles for Rrp1 and Rrp2 in maintaining centromere function by modulating histone dynamics, contributing to the preservation of genome stability during vegetative cell growth. Competing Interests: Competing interestsThe authors declare no competing or financial interests. (© 2020. Published by The Company of Biologists Ltd.) |
| Databáze: | MEDLINE |
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