miRNAs as radio-response biomarkers for breast cancer stem cells.

Autor: Griñán-Lisón C; Biopathology and Regenerative Medicine Institute (IBIMER), Centre for Biomedical Research (CIBM), University of Granada, Granada, Spain.; Instituto de Investigación Biosanitaria ibs.GRANADA, Spain., Olivares-Urbano MA; Department of Radiology and Physical Medicine, University of Granada, Spain., Jiménez G; Biopathology and Regenerative Medicine Institute (IBIMER), Centre for Biomedical Research (CIBM), University of Granada, Granada, Spain.; Instituto de Investigación Biosanitaria ibs.GRANADA, Spain.; Bio-Health Research Foundation of Eastern Andalusia - Alejandro Otero (FIBAO), Granada, Spain., López-Ruiz E; Biopathology and Regenerative Medicine Institute (IBIMER), Centre for Biomedical Research (CIBM), University of Granada, Granada, Spain.; Instituto de Investigación Biosanitaria ibs.GRANADA, Spain.; Department of Health Sciences, University of Jaén, Spain., Del Val C; Department of Artificial Intelligence, University of Granada, Spain., Morata-Tarifa C; Biopathology and Regenerative Medicine Institute (IBIMER), Centre for Biomedical Research (CIBM), University of Granada, Granada, Spain.; Instituto de Investigación Biosanitaria ibs.GRANADA, Spain.; Andalusian Network for Design and Translation of Advanced Therapies, Sevilla, Spain., Entrena JM; Biopathology and Regenerative Medicine Institute (IBIMER), Centre for Biomedical Research (CIBM), University of Granada, Granada, Spain., González-Ramírez AR; Instituto de Investigación Biosanitaria ibs.GRANADA, Spain.; Bio-Health Research Foundation of Eastern Andalusia - Alejandro Otero (FIBAO), Granada, Spain., Boulaiz H; Biopathology and Regenerative Medicine Institute (IBIMER), Centre for Biomedical Research (CIBM), University of Granada, Granada, Spain.; Instituto de Investigación Biosanitaria ibs.GRANADA, Spain.; Department of Human Anatomy and Embryology, Faculty of Medicine, University of Granada, Spain., Zurita Herrera M; Radiation Oncology Department, Virgen de las Nieves University Hospital, Granada, Spain., Núñez MI; Biopathology and Regenerative Medicine Institute (IBIMER), Centre for Biomedical Research (CIBM), University of Granada, Granada, Spain.; Instituto de Investigación Biosanitaria ibs.GRANADA, Spain.; Department of Radiology and Physical Medicine, University of Granada, Spain., Marchal JA; Biopathology and Regenerative Medicine Institute (IBIMER), Centre for Biomedical Research (CIBM), University of Granada, Granada, Spain.; Instituto de Investigación Biosanitaria ibs.GRANADA, Spain.; Department of Human Anatomy and Embryology, Faculty of Medicine, University of Granada, Spain.
Jazyk: angličtina
Zdroj: Molecular oncology [Mol Oncol] 2020 Mar; Vol. 14 (3), pp. 556-570. Date of Electronic Publication: 2020 Feb 06.
DOI: 10.1002/1878-0261.12635
Abstrakt: In breast cancer (BC), the presence of cancer stem cells (CSCs) has been related to relapse, metastasis, and radioresistance. Radiotherapy (RT) is an extended BC treatment, but is not always effective. CSCs have several mechanisms of radioresistance in place, and some miRNAs are involved in the cellular response to ionizing radiation (IR). Here, we studied how IR affects the expression of miRNAs related to stemness in different molecular BC subtypes. Exposition of BC cells to radiation doses of 2, 4, or 6 Gy affected their phenotype, functional characteristics, pluripotency gene expression, and in vivo tumorigenic capacity. This held true for various molecular subtypes of BC cells (classified by ER, PR and HER-2 status), and for BC cells either plated in monolayer, or being in suspension as mammospheres. However, the effect of IR on the expression of eight stemness- and radioresistance-related miRNAs (miR-210, miR-10b, miR-182, miR-142, miR-221, miR-21, miR-93, miR-15b) varied, depending on cell line subpopulation and clinicopathological features of BC patients. Therefore, clinicopathological features and, potentially also, chemotherapy regimen should be both taken into consideration, for determining a potential miRNA signature by liquid biopsy in BC patients treated with RT. Personalized and precision RT dosage regimes could improve the prognosis, treatment, and survival of BC patients.
(© 2020 The Authors. Published by FEBS Press and John Wiley & Sons Ltd.)
Databáze: MEDLINE
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