Total Microfluidic chip for Multiplexed diagnostics (ToMMx).

Autor: Ozen MO; Bio-Acoustic MEMS in Medicine (BAMM) Laboratory, Canary Center at Stanford for Cancer Early Detection, Department of Radiology, School of Medicine Stanford University Palo Alto, CA, 94304, USA; Stanford Cardiovascular Institute, Stanford University School of Medicine, Stanford, CA, 94305, USA., Sridhar K; Bio-Acoustic MEMS in Medicine (BAMM) Laboratory, Canary Center at Stanford for Cancer Early Detection, Department of Radiology, School of Medicine Stanford University Palo Alto, CA, 94304, USA., Ogut MG; Bio-Acoustic MEMS in Medicine (BAMM) Laboratory, Canary Center at Stanford for Cancer Early Detection, Department of Radiology, School of Medicine Stanford University Palo Alto, CA, 94304, USA., Shanmugam A; Bio-Acoustic MEMS in Medicine (BAMM) Laboratory, Canary Center at Stanford for Cancer Early Detection, Department of Radiology, School of Medicine Stanford University Palo Alto, CA, 94304, USA., Avadhani AS; Bio-Acoustic MEMS in Medicine (BAMM) Laboratory, Canary Center at Stanford for Cancer Early Detection, Department of Radiology, School of Medicine Stanford University Palo Alto, CA, 94304, USA., Kobayashi Y; Stanford Cardiovascular Institute, Stanford University School of Medicine, Stanford, CA, 94305, USA., Wu JC; Stanford Cardiovascular Institute, Stanford University School of Medicine, Stanford, CA, 94305, USA; Division of Cardiovascular Medicine, Stanford University School of Medicine, Stanford, CA, 94305, USA., Haddad F; Stanford Cardiovascular Institute, Stanford University School of Medicine, Stanford, CA, 94305, USA; Division of Cardiovascular Medicine, Stanford University School of Medicine, Stanford, CA, 94305, USA., Demirci U; Bio-Acoustic MEMS in Medicine (BAMM) Laboratory, Canary Center at Stanford for Cancer Early Detection, Department of Radiology, School of Medicine Stanford University Palo Alto, CA, 94304, USA; Stanford Cardiovascular Institute, Stanford University School of Medicine, Stanford, CA, 94305, USA. Electronic address: utkan@stanford.edu.
Jazyk: angličtina
Zdroj: Biosensors & bioelectronics [Biosens Bioelectron] 2020 Feb 15; Vol. 150, pp. 111930. Date of Electronic Publication: 2019 Nov 28.
DOI: 10.1016/j.bios.2019.111930
Abstrakt: Microfluidic technologies offer new platforms for biosensing in various clinical and point-of-care (POC) applications. Currently, at the clinical settings, the gold standard diagnostic platforms for multiplexed sensing are multi-step, time consuming, requiring expensive and bulky instruments with a constant need of electricity which makes them unsuitable for resource-limited or POC settings. These technologies are often limited by logistics, costly assays and regular maintenance. Although there have been several attempts to miniaturize these diagnostic platforms, they stand short of batch fabrication and they are dependent on complementary components such as syringe pumps. Here, we demonstrated the development and clinical testing of a disposable, multiplexed sensing device (ToMMx), which is a portable, high-throughput and user-friendly microfluidic platform. It was built with inexpensive plastic materials and operated manually without requiring electrical power and extensive training. We validated this platform in a small cohort of 50 clinical samples from patients with cardiovascular diseases and healthy controls. The platform is rapid and gives quantifiable results with high sensitivity, as low as 5.29 pg/mL, from only a small sample volume (4 μL). ToMMx platform was compared side-by-side with commercial ELISA kits where the total assay time is reduced 15-fold, from 5 h to 20 min. This technology platform is broadly applicable to various diseases with well-known biomarkers in diagnostics and monitoring, especially with potential future impact at the POC settings.
(Copyright © 2019 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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