Resinous adhesive systems differentially affect the expression of cytokines by human monocytes stimulated or not with Streptococcus mutans in vitro.

Autor: Santos MAXO; Graduate Program in Dentistry, Department of Dentistry, Pontifícia Universidade Católica de Minas Gerais, Av. Dom José Gaspar, 500, Belo Horizonte, Minas Gerais, Brazil., Castro HHO; Graduate Program in Dentistry, Department of Dentistry, Pontifícia Universidade Católica de Minas Gerais, Av. Dom José Gaspar, 500, Belo Horizonte, Minas Gerais, Brazil., Magalhães LMD; Department of Morphology, ICB, Universidade Federal de Minas Gerais, Av. Antônio Carlos, 6627, Belo Horizonte, Minas Gerais, Brazil., Dutra WO; Department of Morphology, ICB, Universidade Federal de Minas Gerais, Av. Antônio Carlos, 6627, Belo Horizonte, Minas Gerais, Brazil; Instituto Nacional de Ciência e Tecnologia em Doenças Tropicais - INCT-DT, Av. Antônio Carlos, 6627, Belo Horizonte, Minas Gerais, Brazil., Gollob KJ; Instituto Nacional de Ciência e Tecnologia em Doenças Tropicais - INCT-DT, Av. Antônio Carlos, 6627, Belo Horizonte, Minas Gerais, Brazil; International Research Center, A.C. Camargo Cancer Center, Rua Taguá, 440, São Paulo, São Paulo, Brazil., Guimarães NR; Department of Microbiology, ICB, Universidade Federal de Minas Gerais, Av. Antônio Carlos, 6627, Belo Horizonte, Minas Gerais, Brazil., Magalhães PP; Department of Microbiology, ICB, Universidade Federal de Minas Gerais, Av. Antônio Carlos, 6627, Belo Horizonte, Minas Gerais, Brazil., de Macêdo Farias L; Department of Microbiology, ICB, Universidade Federal de Minas Gerais, Av. Antônio Carlos, 6627, Belo Horizonte, Minas Gerais, Brazil., Horta MCR; Graduate Program in Dentistry, Department of Dentistry, Pontifícia Universidade Católica de Minas Gerais, Av. Dom José Gaspar, 500, Belo Horizonte, Minas Gerais, Brazil., Souza PEA; Graduate Program in Dentistry, Department of Dentistry, Pontifícia Universidade Católica de Minas Gerais, Av. Dom José Gaspar, 500, Belo Horizonte, Minas Gerais, Brazil. Electronic address: pauloalencar@pucminas.br.
Jazyk: angličtina
Zdroj: Archives of oral biology [Arch Oral Biol] 2020 Mar; Vol. 111, pp. 104641. Date of Electronic Publication: 2019 Dec 24.
DOI: 10.1016/j.archoralbio.2019.104641
Abstrakt: Objectives: The polymerization of adhesive systems is incomplete and the residual monomers that have been released have a cytotoxic capacity. Some teeth develop into pulp necrosis after composite resin restorations. Considering frequent pulpal inflammation in response to cariogenic bacteria, substances released from the patches could affect the cells of the inflammatory infiltrate and interfere with the mechanisms of defense against microorganisms and protection of pulpal tissue. The aim of this study was to evaluate the effect of substances released by different resinous adhesive systems on cell viability and cytokine expression by human monocytes stimulated in vitro with Streptococcus mutans.
Design: Peripheral blood mononuclear cells from 10 healthy subjects were stimulated with S. mutans and then incubated with supernatants obtained from the Single Bond Universal (SBU) or Clearfil SE Bond (CSEB) adhesive systems for eight hours. Staining with Annexin V and 7AAD for analysis of apoptosis were performed and detection of monocytes expressing cytokines IL-1α, IL-6, IL-8, IL-10, IL-12 and TNF-α were performed by flow cytometry.
Results: No treatment significantly affected apoptosis in monocytes. SBU supernatant increased the frequency of monocytes expressing IL-8 and decreased the monocytes expressing IL-10. Considering S. mutans-stimulated cells, while SBU increased the frequency of IL-8 + monocytes, CSEB reduced the frequency of IL-6 and TNF-α positive monocytes.
Conclusions: Products released from SBU seem to induce proinflammatory effects on monocytes while those from CSEB show an anti-inflammatory outcome. These effects may interfere in the control of cytokine-mediated immunoinflammatory pulp reactions, both in the presence and absence of stimulation by cariogenic bacteria.
Competing Interests: Declaration of Competing Interest None.
(Copyright © 2019. Published by Elsevier Ltd.)
Databáze: MEDLINE
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