Genome-wide analysis of epigenetic and transcriptional changes associated with heterosis in pigeonpea.

Autor: Sinha P; International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Patancheru, India., Singh VK; International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Patancheru, India.; International Rice Research Institute, South-Asia Hub, Patancheru, India., Saxena RK; International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Patancheru, India., Kale SM; International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Patancheru, India.; The Leibniz Institute of Plant Genetics and Crop Plant Research, Gatersleben, Germany., Li Y; BGI-Shenzhen, Shenzhen, China., Garg V; International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Patancheru, India., Meifang T; BGI-Shenzhen, Shenzhen, China., Khan AW; International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Patancheru, India., Kim KD; University of Georgia, Athens, USA.; Myongji University, Yongin, Republic of Korea., Chitikineni A; International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Patancheru, India., Saxena KB; International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Patancheru, India., Sameer Kumar CV; International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Patancheru, India., Liu X; BGI-Shenzhen, Shenzhen, China., Xu X; BGI-Shenzhen, Shenzhen, China., Jackson S; University of Georgia, Athens, USA., Powell W; Scotland's Rural College (SRUC), Edinburgh, UK., Nevo E; University of Haifa, Haifa, Israel., Searle IR; The University of Adelaide, Adelaide, Australia., Lodha M; Centre for Cellular and Molecular Biology (CSIR), Hyderabad, India., Varshney RK; International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Patancheru, India.
Jazyk: angličtina
Zdroj: Plant biotechnology journal [Plant Biotechnol J] 2020 Aug; Vol. 18 (8), pp. 1697-1710. Date of Electronic Publication: 2020 Feb 03.
DOI: 10.1111/pbi.13333
Abstrakt: Hybrids are extensively used in agriculture to deliver an increase in yield, yet the molecular basis of heterosis is not well understood. Global DNA methylation analysis, transcriptome analysis and small RNA profiling were aimed to understand the epigenetic effect of the changes in gene expression level in the two hybrids and their parental lines. Increased DNA methylation was observed in both the hybrids as compared to their parents. This increased DNA methylation in hybrids showed that majority of the 24-nt siRNA clusters had higher expression in hybrids than the parents. Transcriptome analysis revealed that various phytohormones (auxin and salicylic acid) responsive hybrid-MPV DEGs were significantly altered in both the hybrids in comparison to MPV. DEGs associated with plant immunity and growth were overexpressed whereas DEGs associated with basal defence level were repressed. This antagonistic patterns of gene expression might contribute to the greater growth of the hybrids. It was also noticed that some common as well as unique changes in the regulatory pathways were associated with heterotic growth in both the hybrids. Approximately 70% and 67% of down-regulated hybrid-MPV DEGs were found to be differentially methylated in ICPH 2671 and ICPH 2740 hybrid, respectively. This reflected the association of epigenetic regulation in altered gene expressions. Our findings also revealed that miRNAs might play important roles in hybrid vigour in both the hybrids by regulating their target genes, especially in controlling plant growth and development, defence and stress response pathways. The above finding provides an insight into the molecular mechanism of pigeonpea heterosis.
(© 2020 The Authors. Plant Biotechnology Journal published by Society for Experimental Biology and The Association of Applied Biologists and John Wiley & Sons Ltd.)
Databáze: MEDLINE
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