Formation of Polarized, Functional Artificial Cells from Compartmentalized Droplet Networks and Nanomaterials, Using One-Step, Dual-Material 3D-Printed Microfluidics.

Autor: Li J; Cardiff University School of Pharmacy and Pharmaceutical Sciences Redwood Building, King Edward VII Ave Cardiff CF10 3NB UK.; Cardiff University School of Engineering Queen's Buildings, 14-17 The Parade Cardiff CF24 3AA UK., Baxani DK; Cardiff University School of Pharmacy and Pharmaceutical Sciences Redwood Building, King Edward VII Ave Cardiff CF10 3NB UK., Jamieson WD; Cardiff University School of Pharmacy and Pharmaceutical Sciences Redwood Building, King Edward VII Ave Cardiff CF10 3NB UK., Xu W; Cardiff Business School Cardiff University Aberconway Building, Colum Dr Cardiff CF10 3EU UK., Rocha VG; Cardiff University School of Engineering Queen's Buildings, 14-17 The Parade Cardiff CF24 3AA UK., Barrow DA; Cardiff University School of Engineering Queen's Buildings, 14-17 The Parade Cardiff CF24 3AA UK., Castell OK; Cardiff University School of Pharmacy and Pharmaceutical Sciences Redwood Building, King Edward VII Ave Cardiff CF10 3NB UK.
Jazyk: angličtina
Zdroj: Advanced science (Weinheim, Baden-Wurttemberg, Germany) [Adv Sci (Weinh)] 2019 Oct 24; Vol. 7 (1), pp. 1901719. Date of Electronic Publication: 2019 Oct 24 (Print Publication: 2020).
DOI: 10.1002/advs.201901719
Abstrakt: The bottom-up construction of synthetic cells with user-defined chemical organization holds considerable promise in the creation of bioinspired materials. Complex emulsions, droplet networks, and nested vesicles all represent platforms for the engineering of segregated chemistries with controlled communication, analogous to biological cells. Microfluidic manufacture of such droplet-based materials typically results in radial or axisymmetric structures. In contrast, biological cells frequently display chemical polarity or gradients, which enable the determination of directionality, and inform higher-order interactions. Here, a dual-material, 3D-printing methodology to produce microfluidic architectures that enable the construction of functional, asymmetric, hierarchical, emulsion-based artificial cellular chassis is developed. These materials incorporate droplet networks, lipid membranes, and nanoparticle components. Microfluidic 3D-channel arrangements enable symmetry-breaking and the spatial patterning of droplet hierarchies. This approach can produce internal gradients and hemispherically patterned, multilayered shells alongside chemical compartmentalization. Such organization enables incorporation of organic and inorganic components, including lipid bilayers, within the same entity. In this way, functional polarization, that imparts individual and collective directionality on the resulting artificial cells, is demonstrated. This approach enables exploitation of polarity and asymmetry, in conjunction with compartmentalized and networked chemistry, in single and higher-order organized structures, thereby increasing the palette of functionality in artificial cellular materials.
Competing Interests: The authors declare no conflict of interest.
(© 2019 The Authors. Published by WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim.)
Databáze: MEDLINE
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