Corynebacterium tuberculostearicum, a human skin colonizer, induces the canonical nuclear factor-κB inflammatory signaling pathway in human skin cells.

Autor: Altonsy MO; Division of Dermatology, Department of Medicine, University of Calgary, Calgary, Canada.; Department of Zoology, Faculty of Science, Sohag University, Sohag, Egypt., Kurwa HA; Division of Dermatology, Department of Medicine, University of Calgary, Calgary, Canada., Lauzon GJ; Division of Dermatology, Department of Medicine, University of Calgary, Calgary, Canada., Amrein M; Department of Cell Biology and Anatomy, University of Calgary, Calgary, Canada., Gerber AN; Department of Medicine, National Jewish Health, Denver, Colorado.; Department of Medicine, University of Colorado, Denver, Colorado., Almishri W; Division of Gastroenterology, Department of Medicine, University of Calgary, Calgary, Canada., Mydlarski PR; Division of Dermatology, Department of Medicine, University of Calgary, Calgary, Canada.
Jazyk: angličtina
Zdroj: Immunity, inflammation and disease [Immun Inflamm Dis] 2020 Mar; Vol. 8 (1), pp. 62-79. Date of Electronic Publication: 2020 Jan 07.
DOI: 10.1002/iid3.284
Abstrakt: Introduction: Corynebacterium tuberculostearicum (C. t.) is a ubiquitous bacterium that colonizes human skin. In contrast to other members of the genus Corynebacterium, such as toxigenic Corynebacterium diphtheriae or the opportunistic pathogen Corynebacterium jeikeium, several studies suggest that C. t. may play a role in skin health and disease. However, the mechanisms underlying these effects remain poorly understood.
Methods: To investigate whether C. t. induces inflammatory pathways in primary human epidermal keratinocytes (HEKs) and human cutaneous squamous carcinoma cells (SCCs), cell culture, reverse transcription-polymerase chain reaction (PCR), enzyme-linked immunosorbent assay, immunofluorescence microscopy, Western blot, chromatin immunoprecipitation-PCR, small interfering RNA knockdown and luciferase reporter expression system were used.
Results: Herein, we demonstrate that C. t. upregulates the messenger RNA (mRNA) and protein levels of inflammatory mediators in two human skin cell lines, HEKs and SCCs. We further show activation of the canonical nuclear factor-κB (NF-κB) pathway in response to C. t. infection, including phosphorylation of the inhibitor of κB (IκB), the nuclear translocation of NF-κB subunit (NF-κB-P 65 ) and the recruitment of NF-κB-P 65 and RNA polymerase to the NF-κB response elements at the promoter region of the inflammatory genes. Lastly, the data confirm that C. t.-induced tumor necrosis factor mRNA expression in HEKs is toll-like receptor 2 (TLR 2 ) dependent.
Conclusion: Our results offer a mechanistic model for C. t.-induced inflammation in human keratinocytes via TLR 2 and activation of IκB kinase and downstream signaling through the canonical NF-κB pathway. Relevance to chronic inflammatory diseases of the skin and cutaneous oncology is discussed.
(© 2020 The Authors. Immunity, Inflammation and Disease published by John Wiley & Sons Ltd.)
Databáze: MEDLINE
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