Assessing the stability of historical and desiccated snake venoms from a medically important Ecuadorian collection.

Autor: Almeida JR; Biomolecules Discovery Group, Universidad Regional Amazónica Ikiam, Km 7 Via Muyuna, Tena, Napo, Ecuador. Electronic address: rafael.dealmeida@ikiam.edu.ec., Mendes B; Departamento de Biologia Animal, Instituto de Biologia, Universidade Estadual de Campinas (UNICAMP), Campinas, São Paulo, Brazil., Patiño RSP; Biomolecules Discovery Group, Universidad Regional Amazónica Ikiam, Km 7 Via Muyuna, Tena, Napo, Ecuador., Pico J; Instituto Nacional de Investigación en Salud Pública (INSPI), Guayaquil, Guayaquil, Ecuador., Laines J; Instituto Nacional de Investigación en Salud Pública (INSPI), Guayaquil, Guayaquil, Ecuador., Terán M; Instituto Nacional de Investigación en Salud Pública (INSPI), Guayaquil, Guayaquil, Ecuador., Mogollón NGS; Biomolecules Discovery Group, Universidad Regional Amazónica Ikiam, Km 7 Via Muyuna, Tena, Napo, Ecuador., Zaruma-Torres F; Facultad de Ciencias Química, Universidad de Cuenca, Cuenca, Azuay, Ecuador; Centro de Innovación de la Salud - EUS/EP, Cuenca, Azuay, Ecuador., Caldeira CADS; Centro de Estudos de Biomoléculas Aplicadas a Saúde (CEBio), Fundação Oswaldo Cruz de Rondônia (Fiocruz Rondônia), Porto Velho, RO, Brazil; Programa de Pós-graduação em Biologia Experimental (PGBIOEXP), Universidade Federal de Rondônia (UNIR)., da Silva SL; Facultad de Ciencias Química, Universidad de Cuenca, Cuenca, Azuay, Ecuador; Centro de Innovación de la Salud - EUS/EP, Cuenca, Azuay, Ecuador.
Jazyk: angličtina
Zdroj: Comparative biochemistry and physiology. Toxicology & pharmacology : CBP [Comp Biochem Physiol C Toxicol Pharmacol] 2020 Apr; Vol. 230, pp. 108702. Date of Electronic Publication: 2020 Jan 03.
DOI: 10.1016/j.cbpc.2020.108702
Abstrakt: Bothrops asper and Bothrops atrox are important venomous snakes from Ecuador responsible for the most of ophidic accidents, which in the past were treated with a national polyvant antivenom. For years, the venom pools were collected and stored at room temperature in a laboratory. Taking into account the controversial ability of desiccated samples to retain their biological effects and enzymatic activities, we investigated the biochemical and toxicological properties of venoms after years of storage. The proteomic profiles of historical venoms analyzed by high-performance liquid chromatography and electrophoresis are very similar. The fresh batches of venom were more lethal than those stored for years, just as the initial and current LD 50 values of these samples changed. Significant differences were showed in the myotoxic and hemorrhagic activity of some venom pools, while no significant statistical differences were found for the edema activity. The enzymatic assays revealed a variation in proteolytic activity on azocasein and phospholipase A 2 activity, and low differences were reported for thrombin-like serine protease activity. The maintenance of the proteomic profile and certain toxicological activities convert this venom library in a valuable source for research purposes. Nonetheless, the significative reduction of toxicological activities, such as hemorrhagic activity not feasible using these samples for the antivenom production.
Competing Interests: Declaration of competing interest The authors declare no relevant conflicts of interest concerning the present manuscript.
(Copyright © 2020 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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