Drosophila Morgana is an Hsp90-interacting protein with a direct role in microtubule polymerisation.

Autor:	Palumbo V; Dipartimento di Biologia e Biotecnologie Sapienza, Università di Roma, 00185 Rome, Italy valeria.palumbo@uniroma1.it j.g.wakefield@exeter.ac.uk.; Biosciences/Living Systems Institute, College of Life and Environmental Sciences, University of Exeter, Exeter EX4 4QD, UK., Tariq A; Biosciences/Living Systems Institute, College of Life and Environmental Sciences, University of Exeter, Exeter EX4 4QD, UK., Borgal L; Biosciences/Living Systems Institute, College of Life and Environmental Sciences, University of Exeter, Exeter EX4 4QD, UK., Metz J; Biosciences/Living Systems Institute, College of Life and Environmental Sciences, University of Exeter, Exeter EX4 4QD, UK., Brancaccio M; Dipartimento di Genetica, Biologia e Biochimica, Università di Torino, 10126 Torino, Italy., Gatti M; Dipartimento di Biologia e Biotecnologie Sapienza, Università di Roma, 00185 Rome, Italy.; Istituto di Biologia e Patologia Molecolari del CNR, 00185 Rome, Italy., Wakefield JG; Biosciences/Living Systems Institute, College of Life and Environmental Sciences, University of Exeter, Exeter EX4 4QD, UK valeria.palumbo@uniroma1.it j.g.wakefield@exeter.ac.uk., Bonaccorsi S; Dipartimento di Biologia e Biotecnologie Sapienza, Università di Roma, 00185 Rome, Italy.
Jazyk:	angličtina
Zdroj:	Journal of cell science [J Cell Sci] 2020 Jan 23; Vol. 133 (2). Date of Electronic Publication: 2020 Jan 23.
DOI:	10.1242/jcs.236786
Abstrakt:	Morgana (Mora, also known as CHORD in flies) and its mammalian homologue, called CHORDC1 or CHP1, is a highly conserved cysteine and histidine-rich domain (CHORD)-containing protein that has been proposed to function as an Hsp90 co-chaperone. Morgana deregulation promotes carcinogenesis in both mice and humans while, in Drosophila , loss of mora causes lethality and a complex mitotic phenotype that is rescued by a human morgana transgene. Here, we show that Drosophila Mora localises to mitotic spindles and co-purifies with the Hsp90-R2TP-TTT supercomplex and with additional well-known Hsp90 co-chaperones. Acute inhibition of Mora function in the early embryo results in a dramatic reduction in centrosomal microtubule stability, leading to small spindles nucleated from mitotic chromatin. Purified Mora binds to microtubules directly and promotes microtubule polymerisation in vitro , suggesting that Mora directly regulates spindle dynamics independently of its Hsp90 co-chaperone role. Competing Interests: Competing interestsThe authors declare no competing or financial interests. (© 2020. Published by The Company of Biologists Ltd.)
Databáze:	MEDLINE
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