The Morphology and Phenotype of Monocyte-Macrophages When Cultured on Bionanofilms Substrates with Different Surface Relief Profiles.

Autor: Menzyanova NG; Siberian Federal University, 79, Svobodnyav, Krasnoyarsk 660041, Russia., Pyatina SA; Siberian Federal University, 79, Svobodnyav, Krasnoyarsk 660041, Russia., Shabanov AV; L.V. Kirensky Institute of Physics, Siberian Branch of the Russian Academy of Sciences, 50/38 Akademgorodok, Krasnoyarsk 660036, Russia., Nemtsev IV; Federal Research Center Krasnoyarsk Scientific Center of the Siberian Branch of the Russian Academy of Sciences, 50 Akademgorodok, Krasnoyarsk 660036, Russia., Stolyarov DP; Federal Center for Cardiovascular Surgery, 45 Karaulnaya, Krasnoyarsk 660020, Russia., Dryganov DB; Federal Center for Cardiovascular Surgery, 45 Karaulnaya, Krasnoyarsk 660020, Russia., Sakhnov EV; Federal Center for Cardiovascular Surgery, 45 Karaulnaya, Krasnoyarsk 660020, Russia., Shishatskaya EI; Siberian Federal University, 79, Svobodnyav, Krasnoyarsk 660041, Russia.
Jazyk: angličtina
Zdroj: Biomolecules [Biomolecules] 2019 Dec 30; Vol. 10 (1). Date of Electronic Publication: 2019 Dec 30.
DOI: 10.3390/biom10010065
Abstrakt: The effect of surface relief profiles of alkanoate-based bionanofilms to the monocyte-macrophages (MN-MPhs) from peripheral blood of patients with atherosclerosis was studied in vitro. Patients were subjected to coronary stenting. Cell morphology and phenotype (expression of CD antigens, levels of production of marker cytokines) in vitro were analyzed before and after the installation of stents. It was shown, that the mean square roughness (Rq) of the bionanofilms determined the variability of cell morphology, CD antigens spectraand activity of production interleukins-6 and -10. Also, it was revealed, that the "activity" of the surface topography of biopolymer substrates depends on the functional state of MNs, isolated in different time points: Before and after stenting the ratios of cell morphotypes and production of cytokines in MN-MPhs differed significantly.
Competing Interests: The authors declare no conflict of interest. The funders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript, or in the decision to publish the results.
Databáze: MEDLINE
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