| Autor: |
Rotenberg MY, Elbaz B, Nair V, Schaumann EN, Yamamoto N, Sarma N, Matino L; Tissue Electronics, Center for Advanced Biomaterials for Healthcare , Istituto Italiano di Tecnologia , 80125 Naples , Italy.; Department of Chemical Materials and Industrial Production Engineering , University of Naples Federico II , 80125 Naples , Italy., Santoro F; Tissue Electronics, Center for Advanced Biomaterials for Healthcare , Istituto Italiano di Tecnologia , 80125 Naples , Italy.; Department of Chemical Materials and Industrial Production Engineering , University of Naples Federico II , 80125 Naples , Italy., Tian B |
| Abstrakt: |
Current techniques for intracellular electrical interrogation are limited by substrate-bound devices, technically demanding methods, or insufficient spatial resolution. In this work, we use freestanding silicon nanowires to achieve photoelectric stimulation in myofibroblasts with subcellular resolution. We demonstrate that myofibroblasts spontaneously internalize silicon nanowires and subsequently remain viable and capable of mitosis. We then show that stimulation of silicon nanowires at separate intracellular locations results in local calcium fluxes in subcellular regions. Moreover, nanowire-myofibroblast hybrids electrically couple with cardiomyocytes in coculture, and photostimulation of the nanowires increases the spontaneous activation rate in coupled cardiomyocytes. Finally, we demonstrate that this methodology can be extended to the interrogation of signaling in neuron-glia interactions using nanowire-containing oligodendrocytes. |
