Matrix-assisted laser desorption ionization-time of flight mass spectrometry-based method for accurate discrimination of Staphylococcus schleiferi subspecies.

Autor: Assumpção YM; Universidade Federal Fluminense, Instituto Biomédico, Niterói, RJ (Rua Professor Hernani Melo No 101, São Domingos, Niterói, RJ, CEP: 24210-130, Brazil., Teixeira IM; Universidade Federal Fluminense, Instituto Biomédico, Niterói, RJ (Rua Professor Hernani Melo No 101, São Domingos, Niterói, RJ, CEP: 24210-130, Brazil; Universidade Federal Do Rio De Janeiro, Instituto De Microbiologia Paulo De Góes, Rio De Janeiro, RJ (Av. Carlos Chagas Filho, 373, Bloco I, 2° Andar, CEP 21941-902, Brazil., Paletta ACC; Universidade Federal Fluminense, Instituto Biomédico, Niterói, RJ (Rua Professor Hernani Melo No 101, São Domingos, Niterói, RJ, CEP: 24210-130, Brazil., Ferreira EO; Universidade Federal Do Rio De Janeiro, Instituto De Microbiologia Paulo De Góes, Rio De Janeiro, RJ (Av. Carlos Chagas Filho, 373, Bloco I, 2° Andar, CEP 21941-902, Brazil., Pinto TCA; Universidade Federal Do Rio De Janeiro, Instituto De Microbiologia Paulo De Góes, Rio De Janeiro, RJ (Av. Carlos Chagas Filho, 373, Bloco I, 2° Andar, CEP 21941-902, Brazil., Penna BA; Universidade Federal Fluminense, Instituto Biomédico, Niterói, RJ (Rua Professor Hernani Melo No 101, São Domingos, Niterói, RJ, CEP: 24210-130, Brazil. Electronic address: bpenna@id.uff.br.
Jazyk: angličtina
Zdroj: Veterinary microbiology [Vet Microbiol] 2020 Jan; Vol. 240, pp. 108472. Date of Electronic Publication: 2019 Oct 24.
DOI: 10.1016/j.vetmic.2019.108472
Abstrakt: S. schleiferi is one of the main species isolated from canine otitis externa, pyoderma and from apparently healthy dogs. The species is divided into two subspecies, S. schleiferi schleiferi and S. schleiferi coagulans. MALDI-TOF MS does not distinguish correctly these two subspecies. This study aimed to identify biomarkers that could possibly discriminate Staphylococcus schleiferi subspecies by MALDI-TOF MS. Twelve strains (eight S. schleiferi schleiferi and four S. schleiferi coagulans) were firstly identified. Each isolate was submitted to a protein extraction protocol and subjected to spectrometry on Bruker Microflex LT mass spectrometer. Spectra were analyzed with the BioNumerics software v7.6. Our results showed that spectra clustered according to subspecies, and a set of five MALDI-TOF MS biomarkers were selected to enable the discrimination of S. schleiferi subspecies. In addition, these biomarkers were predicted to represent highly conserved proteins, which could contribute to the identification of subspecies-specific proteins that could be used for improved subspecies identification in clinical samples.
(Copyright © 2019 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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