Epidermal and Dermal Hallmarks of Photoaging are Prevented by Treatment with Night Serum Containing Melatonin, Bakuchiol, and Ascorbyl Tetraisopalmitate: In Vitro and Ex Vivo Studies.

Autor: Narda M; Innovation and Development, ISDIN, Barcelona, Spain. mridvika.narda@isdin.com., Brown A; External Consultant to ISDIN, Barcelona, Spain., Muscatelli-Groux B; MATRISCIENCE SAS, Paris Santé Cochin, Paris, France., Grimaud JA; MATRISCIENCE SAS, Paris Santé Cochin, Paris, France., Granger C; Innovation and Development, ISDIN, Barcelona, Spain.
Jazyk: angličtina
Zdroj: Dermatology and therapy [Dermatol Ther (Heidelb)] 2020 Feb; Vol. 10 (1), pp. 191-202. Date of Electronic Publication: 2020 Jan 03.
DOI: 10.1007/s13555-019-00349-8
Abstrakt: Introduction: Photoaging is a complex process that is chiefly the result of oxidative stress caused by ultraviolet (UV)-generated reactive oxygen species. To counter this process, we developed a 3-in-1 night facial serum (3-in-1 NFS) containing a combination of direct and indirect antioxidants and polyphenols that is designed to attenuate UV-generated free radicals and stimulate dermal protein synthesis. In clinical trials 3-in-1 NFS improved the appearance of photoaged skin. In this study we sought to identify some of the main histologic changes responsible for this.
Methods: We performed an immunolabeling analysis of some of the salient epidermal and dermal proteins in 3-in-1 NFS-treated primary epidermal keratinocytes (HEKs) and dermal fibroblasts (HDFs) in vitro, and in UV-exposed skin explants ex vivo. Numbers of apoptotic sunburn cells following exposure of 3-in-1 NFS-treated skin explants to UV radiation were also determined.
Results: We demonstrate that 3-in-1 NFS increases levels of filaggrin and aquaporin 3 in HEKs, and levels of collagen I and collagen III in HDFs in vitro. Levels of precursor procollagen type I and tropoelastin were increased in ex vivo skin explants. Numbers of apoptotic sunburn cells were significantly reduced in UV-exposed skin explants. These effects were only observed with the combination of ingredients in 3-in-1 NFS, suggesting that they have a synergistic effect on photoaged skin biology.
Conclusion: Our results show that some of the histological hallmarks of photoaging are improved with the use of 3-in-1 NFS.
Databáze: MEDLINE
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