Global shape of Toll activation is determined by wntD enhancer properties.

Autor:	Rahimi N; Department of Molecular Genetics, Weizmann Institute of Science, Rehovot 761001, Israel., Carmon S; Department of Molecular Genetics, Weizmann Institute of Science, Rehovot 761001, Israel., Averbukh I; Department of Molecular Genetics, Weizmann Institute of Science, Rehovot 761001, Israel., Khajouei F; Department of Computer Science, University of Illinois at Urbana-Champaign, Urbana, IL 61801., Sinha S; Department of Computer Science, University of Illinois at Urbana-Champaign, Urbana, IL 61801., Schejter ED; Department of Molecular Genetics, Weizmann Institute of Science, Rehovot 761001, Israel., Barkai N; Department of Molecular Genetics, Weizmann Institute of Science, Rehovot 761001, Israel., Shilo BZ; Department of Molecular Genetics, Weizmann Institute of Science, Rehovot 761001, Israel; benny.shilo@weizmann.ac.il.
Jazyk:	angličtina
Zdroj:	Proceedings of the National Academy of Sciences of the United States of America [Proc Natl Acad Sci U S A] 2020 Jan 21; Vol. 117 (3), pp. 1552-1558. Date of Electronic Publication: 2020 Jan 03.
DOI:	10.1073/pnas.1918268117
Abstrakt:	Buffering variability in morphogen distribution is essential for reproducible patterning. A theoretically proposed class of mechanisms, termed "distal pinning," achieves robustness by combining local sensing of morphogen levels with global modulation of gradient spread. Here, we demonstrate a critical role for morphogen sensing by a gene enhancer, which ultimately determines the final global distribution of the morphogen and enables reproducible patterning. Specifically, we show that, while the pattern of Toll activation in the early Drosophila embryo is robust to gene dosage of its locally produced regulator, WntD, it is sensitive to a single-nucleotide change in the wntD enhancer. Thus, enhancer properties of locally produced WntD directly impinge on the global morphogen profile. Competing Interests: The authors declare no competing interest.
Databáze:	MEDLINE
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