Impact of rearing temperature on the innate antiviral immune response of growth hormone transgenic female triploid Atlantic salmon (Salmo salar).

Autor: Ignatz EH; AquaBounty Canada, 718 Route 310, Fortune, PE, C0A 2B0, Canada; Memorial University, Fisheries and Marine Institute, 155 Ridge Road, St. John's, NL, A1C 5R3, Canada; Memorial University, Department of Ocean Sciences, 0 Marine Lab Road, St. John's, NL, A1C 5S7, Canada. Electronic address: ehignatz@mun.ca., Braden LM; AquaBounty Canada, 718 Route 310, Fortune, PE, C0A 2B0, Canada; Hoplite Laboratory, Department of Pathology and Microbiology, Atlantic Veterinary College, University of Prince Edward Island, 550 University Ave., Charlottetown, PE, C1A 4P3, Canada. Electronic address: lbraden@aquabounty.com., Benfey TJ; University of New Brunswick, Department of Biology, 10 Bailey Drive, Fredericton, NB, E3B 5A3, Canada. Electronic address: benfey@unb.ca., Caballero-Solares A; Memorial University, Department of Ocean Sciences, 0 Marine Lab Road, St. John's, NL, A1C 5S7, Canada. Electronic address: acaballeroso@mun.ca., Hori TS; Center for Aquaculture Technologies Canada, 20 Hope Street, Souris, PE, C0A 2B0, Canada. Electronic address: Tiago@canadiancove.pe.ca., Runighan CD; AquaBounty Canada, 718 Route 310, Fortune, PE, C0A 2B0, Canada. Electronic address: drunighan@aquabounty.com., Fast MD; Hoplite Laboratory, Department of Pathology and Microbiology, Atlantic Veterinary College, University of Prince Edward Island, 550 University Ave., Charlottetown, PE, C1A 4P3, Canada. Electronic address: mfast@upei.ca., Westcott JD; Memorial University, Fisheries and Marine Institute, 155 Ridge Road, St. John's, NL, A1C 5R3, Canada. Electronic address: Jillian.Westcott@mi.mun.ca., Rise ML; Memorial University, Department of Ocean Sciences, 0 Marine Lab Road, St. John's, NL, A1C 5S7, Canada. Electronic address: mrise@mun.ca.
Jazyk: angličtina
Zdroj: Fish & shellfish immunology [Fish Shellfish Immunol] 2020 Feb; Vol. 97, pp. 656-668. Date of Electronic Publication: 2019 Dec 28.
DOI: 10.1016/j.fsi.2019.12.081
Abstrakt: AquAdvantage Salmon (growth hormone transgenic female triploid Atlantic salmon) are a faster-growing alternative to conventional farmed diploid Atlantic salmon. To investigate optimal rearing conditions for their commercial production, a laboratory study was conducted in a freshwater recirculating aquaculture system (RAS) to examine the effect of rearing temperature (10.5 °C, 13.5 °C, 16.5 °C) on their antiviral immune and stress responses. When each temperature treatment group reached an average weight of 800 g, a subset of fish were intraperitoneally injected with either polyriboinosinic polyribocytidylic acid (pIC, a viral mimic) or an equal volume of sterile phosphate-buffered saline (PBS). Blood and head kidney samples were collected before injection and 6, 24 and 48 h post-injection (hpi). Transcript abundance of 7 antiviral biomarker genes (tlr3, lgp2, stat1b, isg15a, rsad2, mxb, ifng) was measured by real-time quantitative polymerase chain reaction (qPCR) on head kidney RNA samples. Plasma cortisol levels from blood samples collected pre-injection and from pIC and PBS groups at 24 hpi were quantified by ELISA. While rearing temperature and treatment did not significantly affect circulating cortisol, all genes tested were significantly upregulated by pIC at all three temperatures (except for tlr3, which was only upregulated in the 10.5 °C treatment). Target gene activation was generally observed at 24 hpi, with most transcript levels decreasing by 48 hpi in pIC-injected fish. Although a high amount of biological variability in response to pIC was evident across all treatments, rearing temperature significantly influenced transcript abundance and/or fold-changes comparing time- and temperature-matched pIC- and PBS-injected fish for several genes (tlr3, lgp2, stat1b, isg15a, rsad2 and ifng) at 24 hpi. As an example, significantly higher fold-changes of rsad2, isg15a and ifng were found in fish reared at 10.5 °C when compared to 16.5 °C. Multivariate analysis confirmed that rearing temperature modulated antiviral immune response. The present experiment provides novel insight into the relationship between rearing temperature and innate antiviral immune response in AquAdvantage Salmon.
Competing Interests: Declaration of competing interest Authors EHI, LMB and CDR were all employed by AquaBounty Canada during the time of the research. EHI performed all data analysis under the direct supervision of JDW and MLR. AquaBounty Canada had no role in the decision to publish this manuscript.
(Copyright © 2019 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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