First inter-laboratory comparison of Echinococcus granulosus sensu lato diagnosis in Latin America.

Autor:	Jercic MI; Instituto de Salud Pública de Chile Instituto de Salud Pública de Chile Santiago de Chile Chile Instituto de Salud Pública de Chile, Santiago de Chile, Chile., Santillan G; Instituto Nacional de Enfermedades Infecciosas 'Carlos G. Malbran,' Instituto Nacional de Enfermedades Infecciosas 'Carlos G. Malbran,' Buenos Aires Argentina Instituto Nacional de Enfermedades Infecciosas 'Carlos G. Malbran,' Buenos Aires, Argentina., Elola S; Comisión Nacional de Zoonosis Comisión Nacional de Zoonosis Montevideo Uruguay Comisión Nacional de Zoonosis, Montevideo, Uruguay., Quispe Paredes W; Instituto Nacional de Salud Instituto Nacional de Salud Lima Peru Instituto Nacional de Salud, Lima, Peru., Conza Blanco LB; Servicio Nacional de Sanidad Agraria Servicio Nacional de Sanidad Agraria Lima Peru Servicio Nacional de Sanidad Agraria, Lima, Peru., Morel N; Comisión Nacional de Zoonosis Comisión Nacional de Zoonosis Montevideo Uruguay Comisión Nacional de Zoonosis, Montevideo, Uruguay., Villegas R; Instituto de Salud Pública de Chile Instituto de Salud Pública de Chile Santiago de Chile Chile Instituto de Salud Pública de Chile, Santiago de Chile, Chile., Molina Flores B; Centro Panamericano de Fiebre Aftosa Pan American Health Organization (PAHO) / World Health Organization (WHO) Rio de Janeiro Brazil Centro Panamericano de Fiebre Aftosa, Pan American Health Organization (PAHO) / World Health Organization (WHO), Rio de Janeiro, Brazil., Gavidia CM; Facultad de Medicina Veterinaria Universidad Nacional Mayor de San Marcos Lima Peru Facultad de Medicina Veterinaria, Universidad Nacional Mayor de San Marcos, Lima, Peru., Cabrera M; Instituto Nacional de Enfermedades Infecciosas 'Carlos G. Malbran,' Instituto Nacional de Enfermedades Infecciosas 'Carlos G. Malbran,' Buenos Aires Argentina Instituto Nacional de Enfermedades Infecciosas 'Carlos G. Malbran,' Buenos Aires, Argentina., Dos Santos AG; Centro Panamericano de Fiebre Aftosa Pan American Health Organization (PAHO) / World Health Organization (WHO) Rio de Janeiro Brazil Centro Panamericano de Fiebre Aftosa, Pan American Health Organization (PAHO) / World Health Organization (WHO), Rio de Janeiro, Brazil., Sanchez-Vazquez MJ; Centro Panamericano de Fiebre Aftosa Pan American Health Organization (PAHO) / World Health Organization (WHO) Rio de Janeiro Brazil Centro Panamericano de Fiebre Aftosa, Pan American Health Organization (PAHO) / World Health Organization (WHO), Rio de Janeiro, Brazil., Maxwell MJ; The Ohio State University The Ohio State University Columbus, Ohio United States of America The Ohio State University, Columbus, Ohio, United States of America., Vigilato MA; PAHO/WHO Country Office PAHO/WHO Country Office San Salvador El Salvador PAHO/WHO Country Office, San Salvador, El Salvador., Larrieu E; Escuela de Veterinaria Universidad Nacional de Rio Negro Choele Choel Argentina Escuela de Veterinaria, Universidad Nacional de Rio Negro, Choele Choel, Argentina., Del Rio Vilas VJ; School of Veterinary Medicine University of Surrey Surrey United Kingdom School of Veterinary Medicine, University of Surrey, Surrey, United Kingdom.
Jazyk:	angličtina
Zdroj:	Revista panamericana de salud publica = Pan American journal of public health [Rev Panam Salud Publica] 2019 Dec 09; Vol. 43, pp. e89. Date of Electronic Publication: 2019 Dec 09 (Print Publication: 2019).
DOI:	10.26633/RPSP.2019.89
Abstrakt:	Objective: To compare the performance of polymerase chain reaction (PCR) and enzyme-linked immunosorbent assay (ELISA) tests for diagnosing Echinococcus granulosus in dog feces among national reference laboratories in Argentina, Chile, Peru, and Uruguay. Methods: National laboratories affiliated with the Ministry of Health/Agriculture of each country exchanged panels of 10 positive/negative samples obtained from their regular national surveillance programs in November 2015 - November 2016. All laboratories applied PCR; two also applied ELISA techniques. Sensitivity and specificity were determined for each laboratory and concordance of results among the laboratories was evaluated by Cohen Kappa coefficient. Results: Poor concordance (3 of 10 paired comparisons had values of Kappa > 0.4), low sensitivity and specificity across all laboratories, and poor performance of both techniques in detecting E. granulosus in canine feces was demonstrated in this study. An ex-post comparison of the laboratories' test protocols showed substantial heterogeneity that could partially explain poor concordance of results. Conclusion: The results underscore the heterogeneity of canine echinococcosis diagnosis across the region and indicate possible sources of variability. Efforts to standardize canine echinococcosis testing must be included in the plan of action for the Regional Initiative for the Control of Cystic Echinococcosis. Future comparisons with fecal samples of known parasite load are needed. Competing Interests: Conflict of interests. None declared.
Databáze:	MEDLINE
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