Longitudinal Transcriptomic, Proteomic, and Metabolomic Analyses of Citrus sinensis (L.) Osbeck Graft-Inoculated with " Candidatus Liberibacter asiaticus".

Autor: Chin EL; Department of Food Science and Technology , University of California, Davis , Davis , California 95616 , United States., Ramsey JS; Emerging Pests and Pathogens Research Unit, Robert W. Holley Center for Agriculture and Health , USDA Agricultural Research Service , Ithaca , New York 14853 , United States.; Boyce Thompson Institute for Plant Research , Ithaca , New York 14853 , United States., Mishchuk DO; Department of Food Science and Technology , University of California, Davis , Davis , California 95616 , United States., Saha S; Boyce Thompson Institute for Plant Research , Ithaca , New York 14853 , United States., Foster E; Contained Research Facility , University of California, Davis , Davis , California 95616 , United States., Chavez JD; Department of Genome Sciences , University of Washington , Seattle , Washington 98195 , United States., Howe K; Emerging Pests and Pathogens Research Unit, Robert W. Holley Center for Agriculture and Health , USDA Agricultural Research Service , Ithaca , New York 14853 , United States.; Boyce Thompson Institute for Plant Research , Ithaca , New York 14853 , United States., Zhong X; Department of Genome Sciences , University of Washington , Seattle , Washington 98195 , United States., Polek M; National Clonal Germplasm Repository for Citrus & Dates , Riverside , California 92507 , United States., Godfrey KE; Contained Research Facility , University of California, Davis , Davis , California 95616 , United States., Mueller LA; Boyce Thompson Institute for Plant Research , Ithaca , New York 14853 , United States., Bruce JE; Department of Genome Sciences , University of Washington , Seattle , Washington 98195 , United States., Heck M; Emerging Pests and Pathogens Research Unit, Robert W. Holley Center for Agriculture and Health , USDA Agricultural Research Service , Ithaca , New York 14853 , United States.; Boyce Thompson Institute for Plant Research , Ithaca , New York 14853 , United States.; Plant Pathology and Plant-Microbe Biology Section, School of Integrative Plant Science , Cornell University , Ithaca , New York 14853 , United States., Slupsky CM; Department of Food Science and Technology , University of California, Davis , Davis , California 95616 , United States.
Jazyk: angličtina
Zdroj: Journal of proteome research [J Proteome Res] 2020 Feb 07; Vol. 19 (2), pp. 719-732. Date of Electronic Publication: 2020 Jan 15.
DOI: 10.1021/acs.jproteome.9b00616
Abstrakt: " Candidatus Liberibacter asiaticus" ( C Las) is the bacterium associated with the citrus disease Huanglongbing (HLB). Current C Las detection methods are unreliable during presymptomatic infection, and understanding C Las pathogenicity to help develop new detection techniques is challenging because C Las has yet to be isolated in pure culture. To understand how C Las affects citrus metabolism and whether infected plants produce systemic signals that can be used to develop improved detection techniques, leaves from Washington Navel orange ( Citrus sinensis (L.) Osbeck) plants were graft-inoculated with C Las and longitudinally studied using transcriptomics (RNA sequencing), proteomics (liquid chromatography-tandem mass spectrometry), and metabolomics (proton nuclear magnetic resonance). Photosynthesis gene expression and protein levels were lower in infected plants compared to controls during late infection, and lower levels of photosynthesis proteins were identified as early as 8 weeks post-grafting. These changes coordinated with higher sugar concentrations, which have been shown to accumulate during HLB. Cell wall modification and degradation gene expression and proteins were higher in infected plants during late infection. Changes in gene expression and proteins related to plant defense were observed in infected plants as early as 8 weeks post-grafting. These results reveal coordinated changes in greenhouse navel leaves during C Las infection at the transcript, protein, and metabolite levels, which can inform of biomarkers of early infection.
Databáze: MEDLINE
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