Validation of methylene blue viability staining with the emerging pathogen Candida auris.

Autor: Parker RA; BioInnovation Laboratory, Kennesaw State University, Kennesaw, GA 30144, USA., Gabriel KT; BioInnovation Laboratory, Kennesaw State University, Kennesaw, GA 30144, USA., Graham K; BioInnovation Laboratory, Kennesaw State University, Kennesaw, GA 30144, USA., Cornelison CT; BioInnovation Laboratory, Kennesaw State University, Kennesaw, GA 30144, USA. Electronic address: ccornel5@kennesaw.edu.
Jazyk: angličtina
Zdroj: Journal of microbiological methods [J Microbiol Methods] 2020 Feb; Vol. 169, pp. 105829. Date of Electronic Publication: 2019 Dec 27.
DOI: 10.1016/j.mimet.2019.105829
Abstrakt: Methylene blue viability staining has been traditionally used to assess viability of Saccharomyces cerevisiae in brewing and wine making. Here, this method was tested and validated with the emerging fungal pathogen Candida auris to determine if this species would also deferentially stain, which could provide utility in assessing microbial control and disinfectant efficacy.
(Copyright © 2019 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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