Hypotensive effect of Eugenia dysenterica leaf extract is primarily related to its vascular action: The possible underlying mechanisms.

Autor: Fidelis-de-Oliveira P; São Paulo State University/UNESP, Department of Physiology, Institute of Biosciences, Botucatu, Brazil. Electronic address: patricia.fidelis@unesp.br., Aparecida-Castro S; São Paulo State University/UNESP, Department of Physiology, Institute of Biosciences, Botucatu, Brazil. Electronic address: samanta-castro@hotmail.com., Silva DB; Federal University of Mato Grosso do Sul/UFMS, Laboratório de Produtos Naturais e Espectrometria de Massas (LAPNEM), Campo Grande, Mato Grosso do Sul, Brazil. Electronic address: denisebrentan@gmail.com., Morais IBM; Federal University of Uberlândia/UFU, Department of Pharmacology, Institute of Biomedical Sciences, Campus Umuarama, Uberlândia, Minas Gerais, Brazil. Electronic address: ingridbmorais@live.com., Miranda VHM; São Paulo State University/UNESP, Department of Physiology, Institute of Biosciences, Botucatu, Brazil. Electronic address: Vhmmiranda85@gmail.com., de Gobbi JI; São Paulo State University/UNESP, Department of Physiology, Institute of Biosciences, Botucatu, Brazil. Electronic address: degobbijuliana@gmail.com., Canabrava HAN; Federal University of Uberlândia/UFU, Department of Pharmacology, Institute of Biomedical Sciences, Campus Umuarama, Uberlândia, Minas Gerais, Brazil. Electronic address: canabrava@ufu.br., Bispo-da-Silva LB; Federal University of Uberlândia/UFU, Department of Pharmacology, Institute of Biomedical Sciences, Campus Umuarama, Uberlândia, Minas Gerais, Brazil. Electronic address: luizbispo@ufu.br.
Jazyk: angličtina
Zdroj: Journal of ethnopharmacology [J Ethnopharmacol] 2020 Apr 06; Vol. 251, pp. 112520. Date of Electronic Publication: 2019 Dec 26.
DOI: 10.1016/j.jep.2019.112520
Abstrakt: Ethnopharmacological Relevance: Eugenia dysenterica (ED) leaves are used in Brazil to treat cardiac diseases; however, there are no scientific data describing the effects of this species on cardiac activity.
Aim of the Study: To investigate the effect of ED aqueous leaf extract (EDLE) on hear rate (HR) and mean arterial pressure (MAP) of anaesthetised rats and its underlying mechanism of action.
Material and Methods: EDLE was analysed, and its proanthocyanidin composition was determined. After performing dose-effect curves for EDLE on HR and MAP, EDLE-induced hypotension was evaluated before and after atropine (AT), L-N(ω)-nitro-L-arginine methyl ester (L-NAME), hexamethonium (HXT), indomethacin (IND), carbenoxolone (CBX), or nifedipine (NFD) administration. The effect of proanthocyanidin-depleted extract (EDLE/P-) was also determined and compared to that of the EDLE with proanthocyanidins.
Results: EDLE decreased the MAP in a dose-dependent manner; HR was decreased only with the highest and most toxic dose. Only CBX and NFD decreased EDLE-induced hypotension. Five polymeric series of proanthocyanidins were identified, which were mainly constituted by procyanidin and prodelphinidin units with B-type linkage and up to 12 flavan-3-ol units. EDLE/P- induced hypotension did not differ from that induced by EDLE.
Conclusions: The cardiovascular effects of EDLE were primarily related to its vascular action. EDLE-induced hypotensive effect appeared to involve L-type calcium channel blockage as well as myoendothelial gap junction signalling. The higher molecular weight proanthocyanidins from EDLE are unlikely to contribute to its cardiovascular effect.
(Copyright © 2020 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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