Intravenous infusion of kisspeptin increased serum luteinizing hormone acutely and decreased serum follicle stimulating hormone chronically in prepubertal bull calves.

Autor: Northup SL; College of Veterinary Medicine, University of Tennessee, Knoxville, TN, USA; Department of Animal Science, University of Tennessee, Knoxville, TN, USA., Coffman EA; School of Veterinary Medicine, Louisiana State University, Baton Rouge, LA, USA., Strickland LG; College of Veterinary Medicine, University of Tennessee, Knoxville, TN, USA; Department of Animal Science, University of Tennessee, Knoxville, TN, USA., Pohler KG; Department of Animal Science, University of Tennessee, Knoxville, TN, USA., Daniel JA; School of Mathematical and Natural Sciences, Berry College, Mount Berry, GA, USA., Whitlock BK; College of Veterinary Medicine, University of Tennessee, Knoxville, TN, USA. Electronic address: bwhitloc@utk.edu.
Jazyk: angličtina
Zdroj: Theriogenology [Theriogenology] 2020 Mar 01; Vol. 144, pp. 1-7. Date of Electronic Publication: 2019 Dec 24.
DOI: 10.1016/j.theriogenology.2019.12.013
Abstrakt: Kisspeptin (KP) is a hypothalamic neuropeptide that stimulates the secretion of gonadotropin releasing hormone. To determine the acute and chronic effects of KP on serum concentrations of luteinizing hormone (LH) and follicle stimulating hormone (FSH), prepubertal bull calves [12 ± 1 (SD) weeks of age; 96.5 ± 14.5 kg BW] were administered one of four treatments [0.0 (control; CON), 0.125 (L-KP), 0.25 (M-KP), or 0.5 (H-KP) μg of KP/kg BW/hour] by intravenous infusion for 76 h. Blood samples were collected every 15 min for the first (acute; 1-6 h; Day 1) and last (chronic; 71-76 h; Day 4) 6 h of the intravenous infusions. Serum concentrations of LH and FSH were determined by radioimmunoassay. For each day, effects of treatment, time, and interactions on LH and FSH concentrations and pulse parameters were analyzed using procedures for repeated measures with JMP Software (SAS Inst. Inc., Cary, NC). There was a treatment effect (P = 0.002) and a treatment × time interaction during Day 1 (P = 0.02) such that LH concentrations were greatest following administration of all doses of KP when compared to CON. However, there was no treatment effect (P = 0.57) or a treatment × time interaction during Day 4 (P = 0.20) on serum LH concentrations. There was a treatment by day interaction (P = 0.02) on mean serum FSH concentrations. Most notably, on Day 4 mean serum FSH concentrations during intravenous infusion of M-KP and H-KP doses were less than that of CON. There was a treatment by day interaction (P = 0.0054) on FSH pulse amplitude concentrations, such that intravenous infusion of all doses of KP on Day 4 decreased FSH pulse amplitudes. In conclusion, acute infusion of KP increased LH concentrations and chronic infusion of KP decreased FSH concentrations. Despite the potential suppression of the hypothalamic-pituitary-gonadal axis with chronic infusion of KP, there are likely applications of KP, KP analogs, or KP receptor agonists to hasten the onset of puberty in livestock.
Competing Interests: Declaration of competing interest The authors declare no potential conflicts of interest with respect to the research, authorship, and/or publication of this article.
(Copyright © 2019 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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