Metagenome sequencing of fingermillet-associated microbial consortia provides insights into structural and functional diversity of endophytes.

Autor: Prasannakumar MK; 1Plant Pathogenomic Laboratory, Department of Plant Pathology, University of Agricultural Sciences, GKVK, Bengaluru, Karnataka India., Mahesh HB; Department of Genetics and Plant Breeding, College of Agriculture, Mandya, Karnataka India., Desai RU; 1Plant Pathogenomic Laboratory, Department of Plant Pathology, University of Agricultural Sciences, GKVK, Bengaluru, Karnataka India., Kunduru B; 1Plant Pathogenomic Laboratory, Department of Plant Pathology, University of Agricultural Sciences, GKVK, Bengaluru, Karnataka India., Narayan KS; 3Department of Centre for Advanced Study in Botany, University of Madras, Chennai, India., Teli K; 1Plant Pathogenomic Laboratory, Department of Plant Pathology, University of Agricultural Sciences, GKVK, Bengaluru, Karnataka India., Puneeth ME; 1Plant Pathogenomic Laboratory, Department of Plant Pathology, University of Agricultural Sciences, GKVK, Bengaluru, Karnataka India., Rajadurai RC; AgriGenome Laboratory, Cochin, Kerala India., Parivallal B; 1Plant Pathogenomic Laboratory, Department of Plant Pathology, University of Agricultural Sciences, GKVK, Bengaluru, Karnataka India., Babu GV; 3Department of Centre for Advanced Study in Botany, University of Madras, Chennai, India.
Jazyk: angličtina
Zdroj: 3 Biotech [3 Biotech] 2020 Jan; Vol. 10 (1), pp. 15. Date of Electronic Publication: 2019 Dec 10.
DOI: 10.1007/s13205-019-2013-0
Abstrakt: Endophytes confer unique ecological advantages to their host plants. In this study, we have characterized the diversity of endophytic consortia associated with the GPU-28 (GPU) and Udurumallige (UM) finger millet varieties, which are resistant and susceptible to the blast disease, respectively. Whole genome metagenome sequencing of GPU and UM helped to identify 1029 species (includes obligate endophytes) of microbiota. Among them, 385 and 357 species were unique to GPU and UM, respectively. Remaining 287 species were common to both the varieties. Actinobacteria and other plant-growth promoting bacteria were abundant in GPU as compared to UM. Functional annotation of genes predicted from genomes of endophytes associated with GPU variety showed that many genes had functional role in stress response, secondary metabolism, aromatic compounds, glutathione, and cysteine synthesis pathways as compared to UM. Based on in vitro and in planta studies, Bacillus cereus and Paenibacillus spp. were found to be effective in suppressing the growth of blast disease pathogen Magnaporthe grisea (strain MG03). In the future, these strains could serve as potential biocontrol agents to reduce the incidence of blast disease in finger millet crop.
Competing Interests: Conflict of interestAuthor Rajadurai, R. C. was employed by company AgriGenome Laboratory. All other authors declare no competing interests.
(© King Abdulaziz City for Science and Technology 2019.)
Databáze: MEDLINE
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