| Autor: |
Costa FPD; Laboratory of Functional and Structural Biology, Institute of Biological Sciences, Federal University of Pará (UFPA), Belém-Pará 66075-110, Brazil.; Laboratory of Tissue Culture and Cytogenetics, Environment Section, Evandro Chagas Institute, Ananindeua-Pará 67030-000, Brazil., Puty B; Laboratory of Functional and Structural Biology, Institute of Biological Sciences, Federal University of Pará (UFPA), Belém-Pará 66075-110, Brazil.; Laboratory of Tissue Culture and Cytogenetics, Environment Section, Evandro Chagas Institute, Ananindeua-Pará 67030-000, Brazil., Nogueira LS; Laboratory of Functional and Structural Biology, Institute of Biological Sciences, Federal University of Pará (UFPA), Belém-Pará 66075-110, Brazil.; Laboratory of Tissue Culture and Cytogenetics, Environment Section, Evandro Chagas Institute, Ananindeua-Pará 67030-000, Brazil., Mitre GP; Laboratory of Cell Culture, Faculty of Dentistry, Federal University of Pará (UFPA), Belém-Pará 66075-110, Brazil., Santos SMD; Laboratory of Oxidative Stress and Clinical Immunology, Faculty of Pharmacy, Federal University of Pará (UFPA), Belém-Pará 66075-110, Brazil., Teixeira BJB; Center for Valorization of Amazonian Bioactive Compounds (CVACBA) & Federal University of Pará UFPA, Belém-Pará 66075-110, Brazil., Kataoka MSDS; Laboratory of Cell Culture, Faculty of Dentistry, Federal University of Pará (UFPA), Belém-Pará 66075-110, Brazil., Martins MD; Department of Oral Pathology, School of Dentistry, Federal University of Rio Grande do Sul, Porto Alegre 91509-900, RS, Brazil., Barboza CAG; Department of Morphology, Federal University of Rio Grande do Norte, Natal 59078-970, Brazil., Monteiro MC; Laboratory of Oxidative Stress and Clinical Immunology, Faculty of Pharmacy, Federal University of Pará (UFPA), Belém-Pará 66075-110, Brazil., Rogez H; Center for Valorization of Amazonian Bioactive Compounds (CVACBA) & Federal University of Pará UFPA, Belém-Pará 66075-110, Brazil., Oliveira EHC; Laboratory of Tissue Culture and Cytogenetics, Environment Section, Evandro Chagas Institute, Ananindeua-Pará 67030-000, Brazil., Lima RR; Laboratory of Functional and Structural Biology, Institute of Biological Sciences, Federal University of Pará (UFPA), Belém-Pará 66075-110, Brazil. |
| Abstrakt: |
Piceatannol is a resveratrol metabolite that is considered a potent antioxidant and cytoprotector because of its high capacity to chelate/sequester reactive oxygen species. In pathogenesis of periodontal diseases, the imbalance of reactive oxygen species is closely related to the disorder in the cells and may cause changes in cellular metabolism and mitochondrial activity, which is implicated in oxidative stress status or even in cell death. In this way, this study aimed to evaluate piceatannol as cytoprotector in culture of human periodontal ligament fibroblasts through in vitro analyses of cell viability and oxidative stress parameters after oxidative stress induced as an injury simulator. Fibroblasts were seeded and divided into the following study groups: control, vehicle, control piceatannol, H 2 O 2 exposure, and H 2 O 2 exposure combined with the maintenance in piceatannol ranging from 0.1 to 20 μM. The parameters analyzed following exposure were cell viability by trypan blue exclusion test, general metabolism status by the 3-[4,5-dimethylthiazole-2-yl]-2,5-diphenyltetrazolium bromide (MTT) method, mitochondrial activity through the ATP production, total antioxidant capacity, and reduced gluthatione. Piceatannol was shown to be cytoprotective due the maintenance of cell viability between 1 and 10 μM even in the presence of H 2 O 2 . In a concentration of 0.1 μM piceatannol decreased significantly cell viability but increased cellular metabolism and antioxidant capacity of the fibroblasts. On the other hand, the fibroblasts treated with piceatannol at 1 μM presented low metabolism and antioxidant capacity. However, piceatannol did not protect cells from mitochondrial damage as measured by ATP production. In summary, piceatannol is a potent antioxidant in low concentrations with cytoprotective capacity, but it does not prevent all damage caused by hydrogen peroxide. |
