Triiodothyronine activated extranuclear pathways upregulate adiponectin and leptin in murine adipocytes.

Autor: Mathias LS; São Paulo State University (UNESP), Botucatu Medical School, Botucatu, São Paulo, Brazil. Electronic address: lucas.mathias@unesp.br., Rodrigues BM; São Paulo State University (UNESP), Botucatu Medical School, Botucatu, São Paulo, Brazil., Gonçalves BM; São Paulo State University (UNESP), Botucatu Medical School, Botucatu, São Paulo, Brazil., Moretto FCF; São Paulo State University (UNESP), Botucatu Medical School, Botucatu, São Paulo, Brazil., Olimpio RMC; São Paulo State University (UNESP), Botucatu Medical School, Botucatu, São Paulo, Brazil., Deprá I; São Paulo State University (UNESP), Botucatu Medical School, Botucatu, São Paulo, Brazil., De Sibio MT; São Paulo State University (UNESP), Botucatu Medical School, Botucatu, São Paulo, Brazil., Tilli HP; São Paulo State University (UNESP), Botucatu Medical School, Botucatu, São Paulo, Brazil., Nogueira CR; São Paulo State University (UNESP), Botucatu Medical School, Botucatu, São Paulo, Brazil., de Oliveira M; São Paulo State University (UNESP), Botucatu Medical School, Botucatu, São Paulo, Brazil.
Jazyk: angličtina
Zdroj: Molecular and cellular endocrinology [Mol Cell Endocrinol] 2020 Mar 01; Vol. 503, pp. 110690. Date of Electronic Publication: 2019 Dec 23.
DOI: 10.1016/j.mce.2019.110690
Abstrakt: Adiponectin and leptin, important for metabolic regulation, are synthesized and secreted by adipose tissue and are influenced by triiodothyronine (T3) that activates the MAPK/ERK and integrin αVβ3 pathways, modulating gene expression. Adipocytes were treated with T3 (10 nM), for 1 h, in the absence or presence of PD98059 (PD) and tetraiodothyroacetic acid (Tetrac), which are pathways inhibitors. The cells were incubated with Adipo Red/Oil Red O reagents, and intracellular lipid accumulation [glycerol and triacylglycerol (TAG)], MTT, 8-hydroxideoxyguanosine (8-OH-dG), and mRNA and protein expression were assessed. T3 increased leptin mRNA and protein expression, and, in contrast, there was a decrease in the Tetrac + T3 group. Adiponectin mRNA expression was not altered by T3, though it had increased its protein expression, which was terminated by inhibitors PD + T3 and Tetrac + T3. However, T3 did not alter PPARγ protein expression, lipid accumulation, TAG, glycerol, and DNA damage, but PD + T3 and Tetrac + T3 reduced these parameters. T3 activated the MAPK/ERK pathway on adipocytes to modulate the adiponectin protein expression and integrin αvβ3 to alter the leptin gene expression.
(Copyright © 2019 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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