Leaky Optoelectrical Fiber for Optogenetic Stimulation and Electrochemical Detection of Dopamine Exocytosis from Human Dopaminergic Neurons.

Autor: Vasudevan S; Department of Biotechnology and Biomedicine (DTU Bioengineering) Technical University of Denmark Produktionstorvet Building 423, Room 122 2800 Kongens Lyngby Denmark., Kajtez J; Department of Biotechnology and Biomedicine (DTU Bioengineering) Technical University of Denmark Produktionstorvet Building 423, Room 122 2800 Kongens Lyngby Denmark., Bunea AI; National Center for Nano Fabrication and Characterization (DTU Nanolab) Technical University of Denmark Ørsteds Plads Building 347 2800 Kongens Lyngby Denmark., Gonzalez-Ramos A; Epilepsy Centre Department of Clinical Sciences Lund University Hospital 221 84 Lund Sweden., Ramos-Moreno T; Lund Stem Cell Center Division of Neurosurgery Department of Clinical Sciences Lund University 221 84 Lund Sweden., Heiskanen A; Department of Biotechnology and Biomedicine (DTU Bioengineering) Technical University of Denmark Produktionstorvet Building 423, Room 122 2800 Kongens Lyngby Denmark., Kokaia M; Epilepsy Centre Department of Clinical Sciences Lund University Hospital 221 84 Lund Sweden., Larsen NB; Department of Health Technology (DTU Health Tech) Technical University of Denmark Produktionstorvet Building 423 2800 Kongens Lyngby Denmark., Martínez-Serrano A; Department of Molecular Biology Universidad Autónoma de Madrid, and Department of Molecular Neuropathology Center of Molecular Biology Severo Ochoa (UAM-CSIC) Nicolás Cabrera 1 28049 Madrid Spain., Keller SS; National Center for Nano Fabrication and Characterization (DTU Nanolab) Technical University of Denmark Ørsteds Plads Building 347 2800 Kongens Lyngby Denmark., Emnéus J; Department of Biotechnology and Biomedicine (DTU Bioengineering) Technical University of Denmark Produktionstorvet Building 423, Room 122 2800 Kongens Lyngby Denmark.
Jazyk: angličtina
Zdroj: Advanced science (Weinheim, Baden-Wurttemberg, Germany) [Adv Sci (Weinh)] 2019 Oct 16; Vol. 6 (24), pp. 1902011. Date of Electronic Publication: 2019 Oct 16 (Print Publication: 2019).
DOI: 10.1002/advs.201902011
Abstrakt: In Parkinson's disease, the degeneration of dopaminergic neurons in substantia nigra leads to a decrease in the physiological levels of dopamine in striatum. The existing dopaminergic therapies effectively alleviate the symptoms, albeit they do not revert the disease progression and result in significant adverse effects. Transplanting dopaminergic neurons derived from stem cells could restore dopamine levels without additional motor complications. However, the transplanted cells disperse in vivo and it is not possible to stimulate them on demand to modulate dopamine release to prevent dyskinesia. In order to address these issues, this paper presents a multifunctional leaky optoelectrical fiber for potential neuromodulation and as a cell substrate for application in combined optogenetic stem cell therapy. Pyrolytic carbon coated optical fibers are laser ablated to pattern micro-optical windows to permit light leakage over a large area. The pyrolytic carbon acts as an excellent electrode for the electrochemical detection of dopamine. Human neural stem cells are genetically modified to express the light sensitive opsin channelrhodopsin-2 and are differentiated into dopaminergic neurons on the leaky optoelectrical fiber. Finally, light leaking from the micro-optical windows is used to stimulate the dopaminergic neurons resulting in the release of dopamine that is detected in real-time using chronoamperometry.
Competing Interests: The authors declare no conflict of interest.
(© 2019 The Authors. Published by WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim.)
Databáze: MEDLINE
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