Morphological and initial  molecular characterization of Clogmia albipunctatus larvae (Diptera: Psychodidae) causing urinary myiasis in Egypt.

Autor: Farrag HMM; Medical Parasitology Department, Faculty of Medicine, Assiut University, Assiut, Egypt., Huseein EAM; Medical Parasitology Department, Faculty of Medicine, Assiut University, Assiut, Egypt., Almatary AM; Medical Parasitology Department, Faculty of Medicine, Assiut University, Assiut, Egypt., Othman RA; Medical Parasitology Department, Faculty of Medicine, Assiut University, Assiut, Egypt.
Jazyk: angličtina
Zdroj: PLoS neglected tropical diseases [PLoS Negl Trop Dis] 2019 Dec 23; Vol. 13 (12), pp. e0007887. Date of Electronic Publication: 2019 Dec 23 (Print Publication: 2019).
DOI: 10.1371/journal.pntd.0007887
Abstrakt: Myiasis is the infestation of human tissues by dipterous fly larvae of the class Insecta. Clogmia albipunctatus, family Psychodidae, is one of the most medically important insects that cause human myiasis. The aim of the present study is the morphological identification and the molecular characterization of moth flies causing many cases of urinary myiasis in Egypt, based on sequencing of the mitochondrial DNA of the larvae. Seven urinary samples of patients complaining of urinary symptoms and giving a history of low socioeconomic level were examined. Recovered larvae were identified using light microscopy and SEM. For molecular identification, the mitochondrial genes Cytochrome B (cytB), NADH1, NADH1, and 16S were sequenced and phylogenetically analyzed. The morphological and molecular characterization could accurately diagnose our patients to have C. albipunctatus infestation. Such results provided the initial set of data on the molecular identification and phylogenetic analysis of moth flies based on DNA barcoding in Egypt.
Competing Interests: The authors have declared that no competing interests exist.
Databáze: MEDLINE
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