Role of spinal adenosine A1 receptors in the analgesic effect of electroacupuncture in a rat model of neuropathic pain.

Autor:	Dai QX; Department of Anesthesiology, The Second Affiliated Hospital of Zhejiang University School of Medicine, Hang Zhou, China.; Department of Anesthesiology, The First Affiliated Hospital of Wen Zhou Medical University, Wen Zhou, China., Huang LP; Department of Anesthesiology, The First Affiliated Hospital of Wen Zhou Medical University, Wen Zhou, China., Mo YC; Department of Anesthesiology, The First Affiliated Hospital of Wen Zhou Medical University, Wen Zhou, China., Yu LN; Department of Anesthesiology, The Second Affiliated Hospital of Zhejiang University School of Medicine, Hang Zhou, China., Du WW; Department of Anesthesiology, The First Affiliated Hospital of Wen Zhou Medical University, Wen Zhou, China., Zhang AQ; Department of Anesthesiology, The First Affiliated Hospital of Wen Zhou Medical University, Wen Zhou, China., Geng WJ; Department of Anesthesiology, The First Affiliated Hospital of Wen Zhou Medical University, Wen Zhou, China., Wang JL; Department of Anesthesiology, The First Affiliated Hospital of Wen Zhou Medical University, Wen Zhou, China., Yan M; Department of Anesthesiology, The Second Affiliated Hospital of Zhejiang University School of Medicine, Hang Zhou, China.
Jazyk:	angličtina
Zdroj:	The Journal of international medical research [J Int Med Res] 2020 Apr; Vol. 48 (4), pp. 300060519883748. Date of Electronic Publication: 2019 Dec 22.
DOI:	10.1177/0300060519883748
Abstrakt:	Objective: The aim of this study was to determine the role of spinal adenosine A1 receptors (A1Rs) in the analgesic effects of electroacupuncture (EA) for neuropathic pain. Methods: We performed EA for 30 minutes at the zusanli acupoint in the legs of rats with previously induced chronic constriction injuries and observed the mechanical and thermal pain thresholds 1 hour later. We also examined adenosine levels by high-performance liquid chromatography and A1R expression in the L4-6 spinal cord by western blot analysis. We then injected A1R short interfering RNA (AV-shA1RNA) into the L4-6 spinal cord to downregulate A1R expression and re-examined the mechanical and thermal pain thresholds. Results: Adenosine levels and A1R expression in the L4-6 spinal cord were increased at 1 hour after EA. In addition, EA exhibited an analgesic effect that was reversed by AV-shA1RNA. Conclusions: Our results suggest that EA at the zusanli acupoint elicits an analgesic effect against neuropathic pain, mediated by A1Rs in the spinal cord.
Databáze:	MEDLINE
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