| Autor: |
Hansen DK; Division of Reproductive and Developmental Toxicology, National Center for Toxicological Research, Jefferson, Arkansas 72079., Anson JF, Hinson WG, Pipkin JL Jr |
| Jazyk: |
angličtina |
| Zdroj: |
Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.) [Proc Soc Exp Biol Med] 1988 Oct; Vol. 189 (1), pp. 136-40. |
| DOI: |
10.3181/00379727-189-1-rc2 |
| Abstrakt: |
Several proteins have been shown to be synthesized in response to various environmental stimuli, including treatment with teratogens. The role of these proteins in the teratogenic process is unknown. Pregnant A/J mice were treated with either a teratogenic or a non-teratogenic dose of the anticonvulsant drug, phenytoin (PHT). Protein synthesis in embryonic craniofacial (target) tissue or forelimb buds (non-target) was determined by incorporation of radiolabeled leucine and analysis by two-dimensional polyacrylamide gel electrophoresis. Synthesis of three proteins in target tissue and one protein in non-target tissue was stimulated by drug treatment. These results suggest that synthesis of specific stress proteins may serve as biomarkers of drug-target tissue interaction. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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