Seminal Plasma Cytokines Are Predictive of the Outcome of Boar Sperm Preservation.

Autor: Barranco I; Department of Medicine and Animal Surgery, Faculty of Veterinary Science, University of Murcia, Murcia, Spain.; Department of Biology, Faculty of Sciences, University of Girona, Girona, Spain., Padilla L; Department of Medicine and Animal Surgery, Faculty of Veterinary Science, University of Murcia, Murcia, Spain., Pérez-Patiño C; Department of Medicine and Animal Surgery, Faculty of Veterinary Science, University of Murcia, Murcia, Spain., Vazquez JM; Department of Medicine and Animal Surgery, Faculty of Veterinary Science, University of Murcia, Murcia, Spain., Martínez EA; Department of Medicine and Animal Surgery, Faculty of Veterinary Science, University of Murcia, Murcia, Spain., Rodríguez-Martínez H; Department of Clinical and Experimental Medicine (IKE), University of Linköping, Linköping, Sweden., Roca J; Department of Medicine and Animal Surgery, Faculty of Veterinary Science, University of Murcia, Murcia, Spain., Parrilla I; Department of Medicine and Animal Surgery, Faculty of Veterinary Science, University of Murcia, Murcia, Spain.
Jazyk: angličtina
Zdroj: Frontiers in veterinary science [Front Vet Sci] 2019 Dec 04; Vol. 6, pp. 436. Date of Electronic Publication: 2019 Dec 04 (Print Publication: 2019).
DOI: 10.3389/fvets.2019.00436
Abstrakt: Background: Boar seminal plasma is rich in cytokines, which could influence the capability of spermatozoa to tolerate preservation. Objectives: To evaluate the involvement of boar seminal plasma cytokines in the changes experienced by boar spermatozoa during their storage, either in liquid or frozen state. Materials and Methods: In two separated experiments, semen samples from healthy and fertile boars were split in two aliquots, one centrifuged twice (1,500 ×g for 10 min) to harvest seminal plasma, whereas the other was either commercially extended (3 × 10 7 sperm/mL) and liquid-stored at 17°C during 144 h ( n = 28, Experiment 1) or frozen-thawed using a standard 0.5 mL protocol ( n = 27, Experiment 2). Sixteen cytokines were quantified using Luminex xMAP®. Sperm attributes (CASA-evaluated total and progressive motility; flow cytometry-evaluated sperm viability, production of intracellular H 2 O 2 and O 2 • - and levels of lipid peroxidation in viable spermatozoa) were evaluated either at 0, 72, or 144 h of liquid storage (Experiment 1) or before freezing and at 30- and 150-min post-thawing (Experiment 2). Results: Multiple linear regression models, with Bayesian approach for variable selection, revealed that the anti-inflammatory TGF-β2, TGF-β3, IL-1Ra, and IL-4 and the pro-inflammatory IL-8 and IL-18, predicted changes in sperm motility for liquid-stored semen while the anti-inflammatory IFN-γ was included in the models predicting changes in all sperm attributes for cryopreserved semen. Conclusion: Specific boar seminal plasma cytokines would contribute to modulate the structural and metabolic changes shown by spermatozoa during preservation, either in liquid or frozen state.
(Copyright © 2019 Barranco, Padilla, Pérez-Patiño, Vazquez, Martínez, Rodríguez-Martínez, Roca and Parrilla.)
Databáze: MEDLINE
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