Lipoic acid nanoforms based on phosphatidylcholine: production and characteristics.

Autor: Shchelkonogov VA; MIREA-Russian Technological University, Moscow, 119571, Russia.; Pirogov Russian National Research Medical University, Russian Ministry of Public Health, Moscow, 117997, Russia., Alyaseva SO; MIREA-Russian Technological University, Moscow, 119571, Russia., Lotosh NY; National Research Center Kurchatov Institute, Moscow, 123182, Russia., Baranova OA; Pirogov Russian National Research Medical University, Russian Ministry of Public Health, Moscow, 117997, Russia., Chekanov AV; Pirogov Russian National Research Medical University, Russian Ministry of Public Health, Moscow, 117997, Russia. avchekanov@mail.ru., Solov'eva EY; Pirogov Russian National Research Medical University, Russian Ministry of Public Health, Moscow, 117997, Russia., Kamyshinskii RA; National Research Center Kurchatov Institute, Moscow, 123182, Russia., Vasilov RG; National Research Center Kurchatov Institute, Moscow, 123182, Russia., Shastina NS; MIREA-Russian Technological University, Moscow, 119571, Russia., Korepanova EA; Pirogov Russian National Research Medical University, Russian Ministry of Public Health, Moscow, 117997, Russia.; Sechenov First Moscow State Medical University (Sechenov University), Moscow, 119991, Russia., Anosov AA; Sechenov First Moscow State Medical University (Sechenov University), Moscow, 119991, Russia., Selishcheva AA; National Research Center Kurchatov Institute, Moscow, 123182, Russia.; Department of Biology, Lomonosov Moscow State University, Moscow, 119234, Russia.; Pirogov Russian National Research Medical University, Russian Ministry of Public Health, Moscow, 117997, Russia.
Jazyk: angličtina
Zdroj: European biophysics journal : EBJ [Eur Biophys J] 2020 Jan; Vol. 49 (1), pp. 95-103. Date of Electronic Publication: 2019 Dec 21.
DOI: 10.1007/s00249-019-01415-x
Abstrakt: We describe the dynamics of lipoic acid (LA) alone, incorporated in liposomes and as a part of nanoemulsions. Mass spectrometry shows that LA in water forms aggregates of two or three molecules in the form of a negatively charged ion and a neutral molecule. Phosphatidylcholine (PC)-based nanoforms of LA as liposomes and nanoemulsions with a particle size equal to 145 nm are characterized by a high degree of incorporation of LA into the nanoparticles and long-term stability during storage at room temperature. Dynamic light scattering (DLS) gives the polydispersity index of the nanoforms (> 0.3), characterizing the homogeneity of the obtained nanodispersions. We found that such emulsions can significantly (5 ×) increase the concentration of LA in the aqueous phase (5-7 mg/mL) when compared with an aqueous solution of LA (1 mg/mL) and by 40% when compared with PC liposomes (4 mg/mL). Moreover, the inclusion of LA in liposomes and nanoemulsions from PC did not change the neutral ζ-potential characteristic of PC nanoforms. CryoTEM established that the structural organization of the liposomes practically did not differ from nanoemulsions and both nanoforms contained both multilayer and single-layer vesicles. When studying the release kinetics of LA from phosphatidylcholine nanoforms, we found that at 22 h, 45-55% of LA was released from nanoparticles, but that at the initial stage of the process LA was slowly released from the nanoemulsions and rapidly from the liposomes. Conductance measurements indicate that LA delivered in all the three forms increase membrane permeability, though this result is most marked with the LA in PC liposomes.
Databáze: MEDLINE
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