Stray dogs in the tropical state of Chiapas, Mexico, harbour atypical and novel genotypes of Toxoplasma gondii.

Autor: Valenzuela-Moreno LF; Laboratorio de Inmunología Experimental, Instituto Nacional de Pediatría, Insurgentes Sur 3700-C, Colonia Insurgentes-Cuicuilco, Delegación Coyoacán, C.P. 04530 Ciudad de México, Mexico; Programa de Doctorado en Ciencias de la Producción y de la Salud Animal, Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Autónoma de México, Av. Universidad 3000, Circuito Exterior S/N Delegación Coyoacán, C.P. 04510 Ciudad Universitaria, Ciudad de México, Mexico., Rico-Torres CP; Laboratorio de Inmunología Experimental, Instituto Nacional de Pediatría, Insurgentes Sur 3700-C, Colonia Insurgentes-Cuicuilco, Delegación Coyoacán, C.P. 04530 Ciudad de México, Mexico., Cedillo-Peláez C; Laboratorio de Inmunología Experimental, Instituto Nacional de Pediatría, Insurgentes Sur 3700-C, Colonia Insurgentes-Cuicuilco, Delegación Coyoacán, C.P. 04530 Ciudad de México, Mexico., Luna-Pastén H; Laboratorio de Inmunología Experimental, Instituto Nacional de Pediatría, Insurgentes Sur 3700-C, Colonia Insurgentes-Cuicuilco, Delegación Coyoacán, C.P. 04530 Ciudad de México, Mexico., Méndez-Cruz ST; Laboratorio de Bioquímica Genética, Instituto Nacional de Pediatría, Insurgentes Sur 3700-C, Colonia Insurgentes-Cuicuilco, Delegación Coyoacán, C.P. 04530 Ciudad de México, Mexico., Reyes-García ME; Laboratorio de Biotecnología de Pequeños Rumiantes y Patología, FMVZ-UNACH, Tuxtla Gutiérrez, Chiapas, Mexico., Correa D; Laboratorio de Inmunología Experimental, Instituto Nacional de Pediatría, Insurgentes Sur 3700-C, Colonia Insurgentes-Cuicuilco, Delegación Coyoacán, C.P. 04530 Ciudad de México, Mexico., Alves BF; Laboratório de Doenças Parasitárias, Departamento de Medicina Veterinária Preventiva e Saúde Animal, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo - USP, São Paulo, SP, Brazil., Pena HFJ; Laboratório de Doenças Parasitárias, Departamento de Medicina Veterinária Preventiva e Saúde Animal, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo - USP, São Paulo, SP, Brazil. Electronic address: hfpena@usp.br., Caballero-Ortega H; Laboratorio de Inmunología Experimental, Instituto Nacional de Pediatría, Insurgentes Sur 3700-C, Colonia Insurgentes-Cuicuilco, Delegación Coyoacán, C.P. 04530 Ciudad de México, Mexico. Electronic address: hcaballero_2000@yahoo.com.mx.
Jazyk: angličtina
Zdroj: International journal for parasitology [Int J Parasitol] 2020 Jan; Vol. 50 (1), pp. 85-90. Date of Electronic Publication: 2019 Dec 19.
DOI: 10.1016/j.ijpara.2019.12.001
Abstrakt: Genotyping of Toxoplasma gondii remains a relevant topic of study, since genotypes can be related to the presentation and severity of toxoplasmosis. To date, 292 restriction fragment length polymorphism genotypes have been described around the world. Serosurveys in southeastern Mexico have documented exposure in over 70% of people and certain animals. Recently, we have described new genotypes and mixed infections in feral cats from Quintana Roo. Thus, the aim of this study was to genotype T. gondii and to describe its genetic variability, from naturally infected stray dogs of Chiapas, which has different geographical and climatic conditions from those found at the Yucatan Peninsula and the other parts of the country. Eleven stray dogs were captured and bled to obtain DNA, and then they were euthanized to perform necropsies and to collect target tissues. Diagnosis of T. gondii was done by quantitative real-time PCR (qPCR) and endpoint PCR. Genotyping was carried out, amplifying 12 polymorphic markers and 15 microsatellites. Atypical SAG3 gene products were cloned and sequenced. All blood samples of dogs were positive to T. gondii DNA by PCR. Two isolates were obtained from pooled heart and diaphragm tissue of two dogs. Two complete PCR-RFLP genotypes were identified (type BrIII and #28). Four animals had mixed infections. A new RFLP atypical allele for the SAG3 marker was observed; cloning and sequencing analysis of this locus revealed mixed infection by a strain identical to GT1, and one type I × II intragenic recombinant. The microsatellite analysis revealed that both isolates are atypical. Thus, atypical new genotypes of T. gondii and mixed infections were found in dogs of Chiapas. The results found here and in genotyping studies in México suggest that the southeastern region favours wide genetic diversity of T. gondii and the possible presence of virulent genotypes such as those found in central and South America.
(Copyright © 2019 Australian Society for Parasitology. Published by Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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