Encapsulated bacteriophages in alginate-nanohydroxyapatite hydrogel as a novel delivery system to prevent orthopedic implant-associated infections.

Autor: Barros JAR; I3S - Instituto de Investigação e Inovação em Saúde, Universidade do Porto, Porto, Portugal; INEB - Instituto de Engenharia Biomédica, Universidade do Porto, Porto, Portugal; FEUP - Faculdade de Engenharia, Universidade do Porto, Porto, Portugal. Electronic address: joana.barros@ineb.up.pt., Melo LDR; Laboratório de Investigação em Biofilmes Rosário Oliveira, Center of Biological Engineering, University of Minho, Braga, Portugal., Silva RARD; Laboratory for Bone Metabolism and Regeneration - Faculty of Dental Medicine, University of Porto, Porto, Portugal; REQUIMTE/LAQV, University of Porto, Porto, Portugal., Ferraz MP; FP-ENAS/CEBIMED - University Fernando Pessoa Energy, Environment and Health Research Unit/Biomedical Research Center, Porto, Portugal., Azeredo JCVR; Laboratório de Investigação em Biofilmes Rosário Oliveira, Center of Biological Engineering, University of Minho, Braga, Portugal., Pinheiro VMC; Department of Animal Sciences, ECAV, University of Trás-os-Montes e Alto Douro, Vila Real, Portugal., Colaço BJA; Department of Animal Sciences, ECAV, University of Trás-os-Montes e Alto Douro, Vila Real, Portugal; Center for the Research and Technology of Agro-Environmental and Biological Sciences, University of Trás-os-Montes e Alto Douro, Vila Real, Portugal., Fernandes MHR; Laboratory for Bone Metabolism and Regeneration - Faculty of Dental Medicine, University of Porto, Porto, Portugal; REQUIMTE/LAQV, University of Porto, Porto, Portugal., Gomes PS; Laboratory for Bone Metabolism and Regeneration - Faculty of Dental Medicine, University of Porto, Porto, Portugal; REQUIMTE/LAQV, University of Porto, Porto, Portugal., Monteiro FJ; I3S - Instituto de Investigação e Inovação em Saúde, Universidade do Porto, Porto, Portugal; INEB - Instituto de Engenharia Biomédica, Universidade do Porto, Porto, Portugal; FEUP - Faculdade de Engenharia, Universidade do Porto, Porto, Portugal.
Jazyk: angličtina
Zdroj: Nanomedicine : nanotechnology, biology, and medicine [Nanomedicine] 2020 Feb; Vol. 24, pp. 102145. Date of Electronic Publication: 2019 Dec 17.
DOI: 10.1016/j.nano.2019.102145
Abstrakt: An innovative delivery system based on bacteriophages-loaded alginate-nanohydroxyapatite hydrogel was developed as a multifunctional approach for local tissue regeneration and infection prevention and control. Bacteriophages were efficiently encapsulated, without jeopardizing phage viability and functionality, nor affecting hydrogel morphology and chemical composition. Bacteriophage delivery occurred by swelling-disintegration-degradation process of the alginate structure and was influenced by environmental pH. Good tissue response was observed following the implantation of bacteriophages-loaded hydrogels, sustaining their biosafety profile. Bacteriophages-loaded hydrogels did not affect osteoblastic cells' proliferation and morphology. A strong osteogenic and mineralization response was promoted through the implantation of hydrogels system with nanohydroxyapatite. Lastly, bacteriophages-loaded hydrogel showed excellent antimicrobial activity inhibiting the attachment and colonization of multidrug-resistant E. faecalis surrounding and within femoral tissues. This new local delivery approach could be a promising approach to prevent and control bacterial contamination during implantation and bone integration.
(Copyright © 2019 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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