Balanced Th1/Th2 immune response induced by MSP1a functional motif coupled to multiwalled carbon nanotubes as anti-anaplasmosis vaccine in murine model.

Autor: Pimentel LS; Laboratory of Nanobiotechnology, Institute of Biotechnology, Federal University of Uberlândia, Uberlândia, MG, Brazil. Electronic address: leticiapimentel@ufu.br., Turini CA; Laboratory of Nanobiotechnology, Institute of Biotechnology, Federal University of Uberlândia, Uberlândia, MG, Brazil., Santos PS; Laboratory of Nanobiotechnology, Institute of Biotechnology, Federal University of Uberlândia, Uberlândia, MG, Brazil., Morais MA; Laboratory of Nanobiotechnology, Institute of Biotechnology, Federal University of Uberlândia, Uberlândia, MG, Brazil., Souza AG; Laboratory of Nanobiotechnology, Institute of Biotechnology, Federal University of Uberlândia, Uberlândia, MG, Brazil., Barbosa MB; Laboratory of Chemistry of Carbon Nanostructures, Nuclear Technology Development Center, CDTN, Belo Horizonte, MG, Brazil., Martins EMDN; Laboratory of Chemistry of Carbon Nanostructures, Nuclear Technology Development Center, CDTN, Belo Horizonte, MG, Brazil., Coutinho LB; Animal Facilities Network (REBIR), Federal University of Uberlândia, Uberlândia, MG, Brazil., Furtado CA; Laboratory of Chemistry of Carbon Nanostructures, Nuclear Technology Development Center, CDTN, Belo Horizonte, MG, Brazil., Ladeira LO; Laboratory of Nanomaterials, Department of Physics, Federal University of Minas Gerais, Belo Horizonte, MG, Brazil., Martins JR; Laboratory of Parasitology, Institute of Veterinary Research Desidério Finamor, Eldorado do Sul, RS, Brazil., Goulart LR; Laboratory of Nanobiotechnology, Institute of Biotechnology, Federal University of Uberlândia, Uberlândia, MG, Brazil., Faria PCB; Laboratory of Nanobiotechnology, Institute of Biotechnology, Federal University of Uberlândia, Uberlândia, MG, Brazil. Electronic address: paulafaria@ufu.br.
Jazyk: angličtina
Zdroj: Nanomedicine : nanotechnology, biology, and medicine [Nanomedicine] 2020 Feb; Vol. 24, pp. 102137. Date of Electronic Publication: 2019 Dec 16.
DOI: 10.1016/j.nano.2019.102137
Abstrakt: Anaplasmosis is one of the most prevalent tick-borne diseases of cattle caused by Anaplasma marginale. MSP1a surface protein has been shown to be involved in eliciting immunity to infected cattle. Carbon nanotubes (CNTs) has been increasingly highlighted due to their needle like structure, which contain multiple attachment sites for biomolecules and may interact with or cross biological membranes, increasing antigen availability to immune system. Here, we have successfully designed a nanocomplex of a synthetic peptide noncovalently attached to multiwalled CNT (MWCNT). Peptide comprising the core motif of the MSP1a was efficiently adsorb onto the nanoparticle surface. The MWCNT-Am1 nanocomplex exhibited high stability and good dispersibility and in vivo immunization showed high levels of IgG1 and IgG2a, followed by increased expression of pro-inflammatory and anti-inflammatory cytokines. This is a proof-of-concept of a nanovaccine that was able to generate a strong immune response compared to the common antigen-adjuvant vaccine without the nanoparticles.
(Copyright © 2019 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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