[CircRNA_005647 inhibits expressions of fibrosis-related genes in mouse cardiac fibroblasts via sponging miR-27b-3p].

Autor:	Yuan S; School of Medicine, South China University of Technology, Guangzhou 510006, China., Liang J; Department of Pharmacy, Jiangmen Central Hospital, Jiangmen 529030, China., Zhang M; Guangdong Cardiovascular Institute//Guangdong Provincial People's Hospital, Guangdong Academy of Medical Sciences, Guangzhou 510080, China., Zhu J; Guangdong Cardiovascular Institute//Guangdong Provincial People's Hospital, Guangdong Academy of Medical Sciences, Guangzhou 510080, China., Pan R; School of Biology and Biological Engineering, South China University of Technology, Guangzhou 510006, China., Li H; Guangdong Cardiovascular Institute//Guangdong Provincial People's Hospital, Guangdong Academy of Medical Sciences, Guangzhou 510080, China., Zeng N; School of Medicine, South China University of Technology, Guangzhou 510006, China., Wen Y; School of Medicine, South China University of Technology, Guangzhou 510006, China., Yi Z; School of Biology and Biological Engineering, South China University of Technology, Guangzhou 510006, China., Shan Z; School of Medicine, South China University of Technology, Guangzhou 510006, China.; Guangdong Cardiovascular Institute//Guangdong Provincial People's Hospital, Guangdong Academy of Medical Sciences, Guangzhou 510080, China.
Jazyk:	čínština
Zdroj:	Nan fang yi ke da xue xue bao = Journal of Southern Medical University [Nan Fang Yi Ke Da Xue Xue Bao] 2019 Nov 30; Vol. 39 (11), pp. 1312-1319.
DOI:	10.12122/j.issn.1673-4254.2019.11.08
Abstrakt:	Objective: To investigate the effect of circRNA_005647 on fibrotic phenotype of mouse cardiac fibroblasts (CFs) and explore its mechanism. Methods: We used an angiotensin Ⅱ (Ang-Ⅱ) capsule pump (daily dose of 1.46 mg/kg for 2 weeks) to establish a mouse model of myocardial fibrosis in C57BL/6 mice. Masson staining was used to detect myocardial fibrosis in the myocardium. The expression of circRNA_005647 in the myocardium of Ang-Ⅱ-infused mice and in Ang-Ⅱ-treated CFs were detected with real-time PCR. Actinomycin D and RNase R exonuclease digestion were used to test the stability of circRNA_005647 in mouse CFs. Over- expression of circRNA_005647 was achieved in the CFs by infecting the cells with a recombinant circRNA_005647 adenovirus (rAd-circRNA_005647), and the expressions of Col1a1, Col3a1 and Acta2 were detected in the cells with real-time PCR and Western blotting. Dual luciferase reporter assay, RNA antisense purification and RNA Pull down assay were performed to identify the interaction between circRNA_005647 and miR-27b-3p. Results: CircRNA_005647 was up- regulated in the myocardium of Ang-Ⅱ- infused mice and in Ang-Ⅱ-treated mouse CFs ( P < 0.01). CircRNA_005647 was more stable than its host gene Myosin IXA ( Myo9a ) in response to actinomycin D ( P < 0.01) and RNase R exonuclease treatment. The expressions of fibrosis-associated genes was down-regulated in the CFs over-expressing circRNA_ 005647 ( P < 0.05). Dual luciferase reporter assay, RNA antisense purification and RNA Pull down assay revealed the interaction between miR-27b-3p and circRNA_005647. MiR-27b-3p obviously enhanced the fibrotic phenotype but reversed the inhibitory effect of circRNA_005647 on the expression of fibrosis associated genes in the CFs ( P < 0.05). Conclusions: CircRNA_005647 is upregulated in cardiac fibrosis and inhibits the expression of fibrosis-related genes through sponging miR-27b-3p in mouse CFs.
Databáze:	MEDLINE
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