A distinct metabolic state arises during the emergence of 2-cell-like cells.

Autor: Rodriguez-Terrones D; Institute of Epigenetics and Stem Cells, Helmholtz Zentrum München, München, Germany., Hartleben G; Institute for Diabetes and Cancer, German Center for Diabetes Research, Helmholtz Zentrum München, Neuherberg, Germany., Gaume X; Institute of Epigenetics and Stem Cells, Helmholtz Zentrum München, München, Germany., Eid A; Institute of Epigenetics and Stem Cells, Helmholtz Zentrum München, München, Germany., Guthmann M; Institute of Epigenetics and Stem Cells, Helmholtz Zentrum München, München, Germany., Iturbide A; Institute of Epigenetics and Stem Cells, Helmholtz Zentrum München, München, Germany., Torres-Padilla ME; Institute of Epigenetics and Stem Cells, Helmholtz Zentrum München, München, Germany.; Faculty of Biology, Ludwig-Maximilians Universität, München, Germany.
Jazyk: angličtina
Zdroj: EMBO reports [EMBO Rep] 2020 Jan 07; Vol. 21 (1), pp. e48354. Date of Electronic Publication: 2019 Dec 18.
DOI: 10.15252/embr.201948354
Abstrakt: Pluripotent stem cells are thought of as a surrogate of early developmental stages that sustain the capacity to generate all cell types in the body, thereby constituting an invaluable tool to address the mechanisms underlying cellular plasticity. In the mouse, cells resembling totipotent 2-cell-stage embryos (2-cell-like cells) arise at a very low frequency in embryonic stem cell (ESC) cultures. However, the extent to which these early-embryonic-like cells recapitulate the molecular features of the early embryo is unclear. Here, we have undertaken a characterization of some of the metabolic features of early-embryonic-like cells in culture. Our data indicate that early-embryonic-like cells exhibit decreased glycolytic and respiratory activity, lower levels of reactive oxygen species and increased glucose uptake, suggesting a shift of the metabolic programme during 2-cell-like cell reprogramming. Accordingly, we find that 2-cell-like cells can be induced by defined metabolites. Thus, in addition to their transcriptional and chromatin features, 2-cell-like cells recapitulate some of the metabolic features of their in vivo counterpart. Altogether, our work underscores a distinct metabolic state of early-embryonic-like cells and identifies compounds that can induce their emergence in vitro.
(© 2019 The Authors. Published under the terms of the CC BY 4.0 license.)
Databáze: MEDLINE
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