Mass Spectrometry Reveals New Insights into the Production of Superoxide Anions and 4-Hydroxynonenal Adducted Proteins in Human Sperm.

Autor: Netherton JK; Department of Biological Science, University of Newcastle, Callaghan, 2308, Australia., Hetherington L; Department of Biological Science, University of Newcastle, Callaghan, 2308, Australia., Ogle RA; Department of Biological Science, University of Newcastle, Callaghan, 2308, Australia., Gavgani MM; Department of Biological Science, University of Newcastle, Callaghan, 2308, Australia., Velkov T; Department of Pharmacology and Therapeutics, University of Melbourne, Parkville, 3010, Australia., Villaverde AIB; Independent researcher, São Paulo, Brazil., Tanphaichitr N; Chronic Disease Program, Ottawa Hospital Research Institute, Department of Obstetrics and Gynaecology and, Department of Biochemistry, Microbiology, Immunology, University of Ottawa, Ottawa, Ontario, K1H 8L6, Canada., Baker MA; Department of Biological Science, University of Newcastle, Callaghan, 2308, Australia.
Jazyk: angličtina
Zdroj: Proteomics [Proteomics] 2020 Jan; Vol. 20 (2), pp. e1900205. Date of Electronic Publication: 2020 Jan 09.
DOI: 10.1002/pmic.201900205
Abstrakt: The free-radical theory of male infertility suggests that reactive oxygen species produced by the spermatozoa themselves are a leading cause of sperm dysfunction, including loss of sperm motility. However, the field is overshadowed on several fronts, primarily because: i) the probes used to measure reactive oxygen species (ROS) are imprecise; and ii) many reports suggesting that oxygen radicals are detrimental to sperm function add an exogenous source of ROS. Herein, a more reliable approach to measure superoxide anion production by human spermatozoa based on MS analysis is used. Furthermore, the formation of the lipid-peroxidation product 4-hydroxynonenal (4-HNE) during in vitro incubation using proteomics is also investigated. The data demonstrate that neither superoxide anion nor other free radicals that cause 4-HNE production are related to the loss of sperm motility during incubation. Interestingly, it appears that many of the 4-HNE adducted proteins, found within spermatozoa, originate from the prostate. A quantitative SWATH analysis demonstrate that these proteins transiently bind to sperm and are then shed during in vitro incubation. These proteomics-based findings propose a revised understanding of oxidative stress within the male reproductive tract.
(© 2019 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.)
Databáze: MEDLINE
Externí odkaz: