Tissue-dependent differences in Bardet-Biedl syndrome gene expression.

Autor: Patnaik SR; Cilia Cell Biology, Institute of Molecular Physiology, Johannes-Gutenberg University, Mainz, 55128, Germany., Farag A; Cilia Cell Biology, Institute of Molecular Physiology, Johannes-Gutenberg University, Mainz, 55128, Germany., Brücker L; Cilia Cell Biology, Institute of Molecular Physiology, Johannes-Gutenberg University, Mainz, 55128, Germany., Volz AK; Cilia Cell Biology, Institute of Molecular Physiology, Johannes-Gutenberg University, Mainz, 55128, Germany., Schneider S; Cilia Cell Biology, Institute of Molecular Physiology, Johannes-Gutenberg University, Mainz, 55128, Germany., Kretschmer V; Cilia Cell Biology, Institute of Molecular Physiology, Johannes-Gutenberg University, Mainz, 55128, Germany., May-Simera HL; Cilia Cell Biology, Institute of Molecular Physiology, Johannes-Gutenberg University, Mainz, 55128, Germany.
Jazyk: angličtina
Zdroj: Biology of the cell [Biol Cell] 2020 Feb; Vol. 112 (2), pp. 39-52. Date of Electronic Publication: 2020 Jan 13.
DOI: 10.1111/boc.201900077
Abstrakt: Background Information: Primary cilia are highly conserved multifunctional cell organelles that extend from the cell membrane. A range of genetic disorders, collectively termed ciliopathies, is attributed to primary cilia dysfunction. The archetypical ciliopathy is the Bardet-Biedl syndrome (BBS), patients of which display virtually all symptoms associated with dysfunctional cilia. The primary cilium acts as a sensory organelle transmitting intra- and extracellular signals thereby transducing various signalling pathways facilitated by the BBS proteins. Growing evidence suggests that cilia proteins also have alternative functions in ciliary independent mechanisms, which might be contributing to disease etiology.
Results: In an attempt to gain more insight into possible differences in organ specific roles, we examined whether relative gene expression for individual Bbs genes was constant across different tissues in mouse, in order to distinguish possible differences in organ specific roles. All tested tissues show differentially expressed Bbs transcripts with some tissues showing a more similar stoichiometric composition of transcripts than others do.  However, loss of Bbs6 or Bbs8 affects expression of other Bbs transcripts in a tissue-dependent way.
Conclusions and Significance: Our data support the hypothesis that in some organs, BBS proteins not only function in a complex but might also have alternative functions in a ciliary independent context. This significantly alters our understanding of disease pathogenesis and development of possible treatment strategies.
(© 2019 Société Française des Microscopies and Société de Biologie Cellulaire de France. Published by John Wiley & Sons Ltd.)
Databáze: MEDLINE
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