Novel ovarian cancer maintenance therapy targeted at mortalin and mutant p53.

Autor: Ramraj SK; Stephenson Cancer Center, The University of Oklahoma Health Sciences Center, Oklahoma City, OK., Elayapillai SP; Stephenson Cancer Center, The University of Oklahoma Health Sciences Center, Oklahoma City, OK., Pelikan RC; Genes and Human Disease Research Program, Oklahoma Medical Research Foundation, Oklahoma City, OK., Zhao YD; Biostatistics & Epidemiology, The University of Oklahoma Health Sciences Center, Oklahoma City, OK., Isingizwe ZR; Department of Pharmaceutical Sciences, College of Pharmacy, The University of Oklahoma Health Sciences Center, Oklahoma City, OK., Kennedy AL; Department of Pathology, College of Medicine, The University of Oklahoma Health Sciences Center, Oklahoma City, OK., Lightfoot SA; Center for Cancer Prevention and Drug Development, Stephenson Cancer Center, The University of Oklahoma Health Sciences Center, Oklahoma City, OK., Benbrook DM; Stephenson Cancer Center, The University of Oklahoma Health Sciences Center, Oklahoma City, OK.; Center for Cancer Prevention and Drug Development, Stephenson Cancer Center, The University of Oklahoma Health Sciences Center, Oklahoma City, OK.; Obstetrics and Gynecologic, College of Medicine, The University of Oklahoma Health Sciences Center, Oklahoma City, OK.
Jazyk: angličtina
Zdroj: International journal of cancer [Int J Cancer] 2020 Aug 15; Vol. 147 (4), pp. 1086-1097. Date of Electronic Publication: 2020 Jan 08.
DOI: 10.1002/ijc.32830
Abstrakt: Current ovarian cancer maintenance therapy is limited by toxicity and no proven impact on overall survival. To study a maintenance strategy targeted at missense mutant p53, we hypothesized that the release of mutant p53 from mortalin inhibition by the SHetA2 drug combined with reactivation of mutant p53 with the PRIMA-1 MET drug inhibits growth and tumor establishment synergistically in a mutant-p53 dependent manner. The Cancer Genome Atlas (TCGA) data and serous ovarian tumors were evaluated for TP53 and HSPA9/mortalin status. SHetA2 and PRIMA-1 MET were tested in ovarian cancer cell lines and fallopian tube secretory epithelial cells using isobolograms, fluorescent cytometry, Western blots and ELISAs. Drugs were administered to mice after peritoneal injection of MESOV mutant p53 ovarian cancer cells and prior to tumor establishment, which was evaluated by logistic regression. Fifty-eight percent of TP53 mutations were missense and there were no mortalin mutations in TCGA high-grade serous ovarian cancers. Mortalin levels were sequentially increased in serous benign, borderline and carcinoma tumors. SHetA2 caused p53 nuclear and mitochondrial accumulation in cancer, but not in healthy, cells. Endogenous or exogenous mutant p53 increased SHetA2 resistance. PRIMA-1 MET decreased this resistance and interacted synergistically with SHetA2 in mutant and wild type p53-expressing cell lines in association with elevated reactive oxygen species/ATP ratios. Tumor-free rates in animals were 0% (controls), 25% (PRIMA1 MET ), 42% (SHetA2) and 67% (combination). SHetA2 (p = 0.004) and PRIMA1 MET (p = 0.048) functioned additively in preventing tumor development with no observed toxicity. These results justify the development of SHetA2 and PRIMA-1 MET alone and in combination for ovarian cancer maintenance therapy.
(© 2019 UICC.)
Databáze: MEDLINE
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