Exposure Effects of Terahertz Waves on Primary Neurons and Neuron-like Cells Under Nonthermal Conditions.

Autor:	Tan SZ; Beijing Institute of Radiation Medicine, Beijing 100850, China., Tan PC; School of Pharmaceutical Sciences, Tsinghua University, Beijing 100084, China., Luo LQ; School of Pharmaceutical Sciences, Tsinghua University, Beijing 100084, China., Chi YL; Inner Mongolia University for the Nationalities, Yakeshi 022150, Inner Mongolia, China., Yang ZL; General Hospital of Northern Theater Command, Shenyang 110000, Liaoning, China., Zhao XL; Beijing Institute of Radiation Medicine, Beijing 100850, China., Zhao L; Beijing Institute of Radiation Medicine, Beijing 100850, China., Dong J; Beijing Institute of Radiation Medicine, Beijing 100850, China., Zhang J; Beijing Institute of Radiation Medicine, Beijing 100850, China., Yao BW; Beijing Institute of Radiation Medicine, Beijing 100850, China., Xu XP; Beijing Institute of Radiation Medicine, Beijing 100850, China., Tian G; State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, China., Chen JK; Fifth Medical Center, Chinese People's Liberation Army General Hospital, Beijing 100071, China., Wang H; Beijing Institute of Radiation Medicine, Beijing 100850, China., Peng RY; Beijing Institute of Radiation Medicine, Beijing 100850, China.
Jazyk:	angličtina
Zdroj:	Biomedical and environmental sciences : BES [Biomed Environ Sci] 2019 Oct; Vol. 32 (10), pp. 739-754.
DOI:	10.3967/bes2019.094
Abstrakt:	Objective: This study aimed to explore the potential effects of terahertz (THz) waves on primary cultured neurons from 4 rat brain regions (hippocampus, cerebral cortex, cerebellum, and brainstem) and 3 kinds of neuron-like cells (MN9D, PC12, and HT22 cells) under nonthermal conditions. Methods: THz waves with an output power of 50 (0.16 THz) and 10 (0.17 THz) mW with exposure times of 6 and 60 min were used in this study. Analysis of temperature change, neurite growth, cell membrane roughness, micromorphology, neurotransmitters and synaptic-related proteins (SYN and PSD95) was used to evaluate the potential effects. Results: Temperature increase caused by the THz wave was negligible. THz waves induced significant neurotransmitter changes in primary hippocampal, cerebellar, and brainstem neurons and in MN9D and PC12 cells. THz wave downregulated SYN expression in primary hippocampal neurons and downregulated PSD95 expression in primary cortical neurons. Conclusion: Different types of cells responded differently after THz wave exposure, and primary hippocampal and cortical neurons and MN9D cells were relatively sensitive to the THz waves. The biological effects were positively correlated with the exposure time of the THz waves. (Copyright © 2019 The Editorial Board of Biomedical and Environmental Sciences. Published by China CDC. All rights reserved.)
Databáze:	MEDLINE
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