RNA disruption indicates CHOP therapy efficacy in canine lymphoma.

Autor: Parissenti AM; Departments of Biology, Chemistry and Biochemistry, Laurentian University, 935 Ramsey Lake Rd., Sudbury, ON, P3E 2C6, Canada. aparissenti@rnadiagnostics.com.; Rna Diagnostics, c/o Health Sciences North Research Institute, 2nd Floor North, 56 Walford Road, Sudbury, ON, P3E 2H3, Canada. aparissenti@rnadiagnostics.com., Pritzker LB; Rna Diagnostics, 21 St. Clair Avenue East, Suite 701, Toronto, ON, M4T 1L9, Canada., Guo B; Rna Diagnostics, c/o Health Sciences North Research Institute, 2nd Floor North, 56 Walford Road, Sudbury, ON, P3E 2H3, Canada., Narendrula R; Rna Diagnostics, c/o Health Sciences North Research Institute, 2nd Floor North, 56 Walford Road, Sudbury, ON, P3E 2H3, Canada., Wang SX; Rna Diagnostics, 21 St. Clair Avenue East, Suite 701, Toronto, ON, M4T 1L9, Canada., Lin LL; Rna Diagnostics, 21 St. Clair Avenue East, Suite 701, Toronto, ON, M4T 1L9, Canada., Pei J; Rna Diagnostics, 21 St. Clair Avenue East, Suite 701, Toronto, ON, M4T 1L9, Canada., Skowronski K; Department of Clinical Studies, Ontario Veterinary College, University of Guelph, 50 Stone Road East, Guelph, ON, N1G 2W1, Canada., Bienzle D; Department of Pathobiology, Ontario Veterinary College, University of Guelph, 50 Stone Road East, Guelph, ON, N1G 2W1, Canada., Woods JP; Department of Clinical Studies, Ontario Veterinary College, University of Guelph, 50 Stone Road East, Guelph, ON, N1G 2W1, Canada., Pritzker KPH; Rna Diagnostics, 21 St. Clair Avenue East, Suite 701, Toronto, ON, M4T 1L9, Canada., Coomber BL; Department of Biomedical Sciences, Ontario Veterinary College, University of Guelph, 50 Stone Road East, Guelph, ON, N1G 2W1, Canada.
Jazyk: angličtina
Zdroj: BMC veterinary research [BMC Vet Res] 2019 Dec 16; Vol. 15 (1), pp. 453. Date of Electronic Publication: 2019 Dec 16.
DOI: 10.1186/s12917-019-2189-x
Abstrakt: Background: Assessment of the efficacy of a multi-agent chemotherapy protocol in which cyclophosphamide, doxorubicin, vincristine and prednisone (CHOP) are administered in canine lymphoma is generally performed by physical measurement of lymph node diameter. However, no consistent correlation has been made with prognostic indicators and the length or absence of clinical remission based on lymph node size. RNA disruption measured mid-therapy has been correlated with increased disease-free survival in recent studies of human cancer and was assessed in this study of canine lymphoma patients. Fine needle aspirate samples were taken before treatment and at weeks 3, 6, and 11 of CHOP therapy. RNA was isolated from these samples and assessed using an Agilent Bioanalyzer. RNA disruption assay (RDA) analysis was performed on the data from the resulting electropherograms.
Results: An increased RNA disruption index (RDI) score was significantly associated with improved progression-free survival.
Conclusions: Predicting the risk of early relapse during chemotherapy could benefit veterinary patients by reducing ineffective treatment and could allow veterinary oncologists to switch earlier to a more effective drug regimen.
Databáze: MEDLINE
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