Fecal indicator bacteria, fecal source tracking markers, and pathogens detected in two Hudson River tributaries.

Autor: Brooks YM; Department of Civil and Environmental Engineering, Cornell University, Ithaca, NY, 14853, USA., Spirito CM; Department of Civil and Environmental Engineering, Cornell University, Ithaca, NY, 14853, USA., Bae JS; Department of Civil and Environmental Engineering, Cornell University, Ithaca, NY, 14853, USA., Hong A; Department of Civil and Environmental Engineering, Cornell University, Ithaca, NY, 14853, USA., Mosier EM; Department of Civil and Environmental Engineering, Cornell University, Ithaca, NY, 14853, USA., Sausele DJ; Department of Civil and Environmental Engineering, Cornell University, Ithaca, NY, 14853, USA., Fernandez-Baca CP; Department of Civil and Environmental Engineering, Cornell University, Ithaca, NY, 14853, USA., Epstein JL; Riverkeeper Inc, 20 Secor Road, Ossining, NY, 10562, USA., Shapley DJ; Riverkeeper Inc, 20 Secor Road, Ossining, NY, 10562, USA., Goodman LB; Population Medicine and Diagnostic Sciences, Cornell University Animal Health Diagnostic Center, Ithaca, NY, 14853, USA., Anderson RR; Population Medicine and Diagnostic Sciences, Cornell University Animal Health Diagnostic Center, Ithaca, NY, 14853, USA., Glaser AL; Population Medicine and Diagnostic Sciences, Cornell University Animal Health Diagnostic Center, Ithaca, NY, 14853, USA., Richardson RE; Department of Civil and Environmental Engineering, Cornell University, Ithaca, NY, 14853, USA. Electronic address: rer26@cornell.edu.
Jazyk: angličtina
Zdroj: Water research [Water Res] 2020 Mar 15; Vol. 171, pp. 115342. Date of Electronic Publication: 2019 Nov 25.
DOI: 10.1016/j.watres.2019.115342
Abstrakt: Volunteer monitoring in the Hudson River watershed since 2012 has identified that the Wallkill River and Rondout Creek tributary complex have elevated concentrations of the fecal indicator bacteria, enterococci. Concentrations of enterococci do not provide insight into the sources of pollution and are imperfect indicators of health risks. In 2017, the regular monthly volunteer monitoring campaign for culturable enterococci at 24 sites on the Wallkill and Rondout expanded to include: (1) culturable measurements of E. coli and quantification of E. coli and Enterococcus specific markers vis nanoscale qPCR, (2) microbial source tracking (MST) assays (avian, human, bovine, and equine) via real time PCR and nanoscale qPCR, and 3) quantification of 12 gastrointestinal pathogens including viruses, bacteria, and protozoa via nanoscale qPCR. Three human associated MST markers (HumM2, HF183, and B. theta) corroborated that human pollution was present in Rondout Creek and widespread in the Wallkill River. The presence of B. theta was associated with increased concentrations of culturable E. coli. Genes for adenovirus 40 and 41 conserved region, rotavirus A NSP3, E. coli eae and stx1, and Giardia lamblia 18S rRNA were detected in >45% of samples. Abundance of rotavirus A NSP3 genes was significantly correlated to the bovine marker gene, CowM3, though wild bird sources cannot be ruled out. This is the first study to investigate potential fecal pollution sources and pathogen concentrations in Hudson tributaries during the months of peak recreational use.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2019. Published by Elsevier Ltd.)
Databáze: MEDLINE
Externí odkaz: